Anti-Cytokeratin 18 antibody [DC 10]

• 产品名称

  Anti-Cytokeratin 18抗体[DC 10]
  参阅全部 Cytokeratin 18 一抗

• 描述

  小鼠单克隆抗体[DC 10] to Cytokeratin 18

• 宿主

  Mouse

• 特异性

  Specific reactivity for the cytokeratin 18 (45 kD) polypeptide.

• 经测试应用

  适用于: IHC-P, ICC/IF, Flow Cyt (Intra), WBmore details

• 种属反应性

  与反应: Human
  不与反应: Mouse, Rat, Sheep, Hamster, Cow, Dog, Pig

• 免疫原

  Tissue, cells or virus corresponding to Human Cytokeratin 18. PMC-42 human breast carcinoma cells.

• 阳性对照

• ICC/IF: Hep-2 cells. IHC-P: Human colon carcinoma. WB: HeLa, HT-29 cell lysate. Flow Cyt (Intra): MCF-7 cells; HeLa cells.

• 常规说明

  This antibody reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid.

   

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

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• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.40
  Preservative: 0.097% Sodium azide
  Constituent: PBS

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Protein A purified

• 纯化说明

  Purified from TCS

• Primary antibody说明

  This antibody reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid.

• 克隆

  单克隆

• 克隆编号

  DC 10

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab7797于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 3875 Human

• Omim: 148070 Human

• SwissProt: P05783 Human

• Unigene: 406013 Human

• 别名

• CK 18 antibody

• CK-18 antibody

• CK18 antibody

• CYK 18 antibody

• CYK18 antibody

• Cytokeratin 18 antibody

• Cytokeratin endo B antibody

• Cytokeratin-18 antibody

• K 18 antibody

• K18 antibody

• K1C18_HUMAN antibody

• KA18 antibody

• Keratin 18 antibody

• Keratin 18, type I antibody

• Keratin D antibody

• keratin, type I cytoskeletal 18 antibody

• Keratin-18 antibody

• Krt18 antibody

• Cell proliferation inducing gene 46 protein antibody

• Cell proliferation inducing protein 46 antibody

• Cell proliferation-inducing gene 46 protein antibody

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 18 antibody [DC 10] (ab7797)

  Immunocytochemistry staining of Cytokeratin 18 in Hep-2 cells using mouse monoclonal antibody DC-10 (ab7797, diluted 1/400), detected with GAM IgG-Alexa Fluor®488 (diluted 1/200; green), cell nuclei stained with PI (1 µg/ml; orange).

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [DC 10] (ab7797)

  Immunohistochemistry staining of human colon (paraffin sections) using ab7797 10 μg/ml

• 

  Western blot - Anti-Cytokeratin 18 antibody [DC 10] (ab7797)

  All lanes : Anti-Cytokeratin 18 antibody [DC 10] (ab7797) at 2 µg/ml
  
  Lane 1 : HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate (Non-Reducing)
  Lane 2 : MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate (Non-Reducing)
  Lane 3 : HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate (Reducing)
  Lane 4 : MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate (Reducing)
  
  Secondary
  All lanes : IRDye800-conjugated anti-mouse secondary antibody.
  
  
  

  A specific band was detected for cytokeratin 18 at approximately 46 kDa, and its proteolytic fragment at approximately 25 kDa.

• 

  Western blot - Anti-Cytokeratin 18 antibody [DC 10] (ab7797)

  Anti-Cytokeratin 18 antibody [DC 10] (ab7797) at 1 µg/ml + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
  
  Observed band size: 40 kDawhy is the actual band size different from the predicted?
  
  

• 

  Flow Cytometry (Intracellular) - Anti-Cytokeratin 18 antibody [DC 10] (ab7797)

  Overlay histogram showing MCF-7 cells stained with ab7797 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab7797, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF-7 cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Triton used under the same conditions.

   

• 

  Flow Cytometry (Intracellular) - Anti-Cytokeratin 18 antibody [DC 10] (ab7797)

  Flow cytometry analysis (intracellular staining) of MCF-7 cells (red-filled) stained using ab7797 anti-cytokeratin 18 purified antibody (concentration in sample 0,6 μg/ml, GAM APC). Isotype control: Caco-2 cells (black-dashed).