Anti-Cytokeratin 19 antibody [BA-17]

• 产品名称

  Anti-Cytokeratin 19抗体[BA-17]
  参阅全部 Cytokeratin 19 一抗

• 描述

  小鼠单克隆抗体[BA-17] to Cytokeratin 19

• 宿主

  Mouse

• 特异性

  Cytokeratin peptide 19 (40 kDa) in human tissue.

• 经测试应用

  适用于: Flow Cyt (Intra), ICC/IF, WB, IHC-P, IPmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Tissue, cells or virus corresponding to Human Cytokeratin 19. Mammary organoids
  Database link: P08727

• 阳性对照

• ICC/IF KO: HepG2, MCF7 cells (MCF7-KRT19 KO used as a negative cell line). WB: MCF-7, HepG2, SW480, MDA-MB-361 cell lysates. IHC-P: Human skin. IP: HepG2 cell extract. Flow Cyt (Intra): MCF7 cells.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.40
  Preservative: 0.097% Sodium azide
  Constituent: PBS

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Protein A purified

• 克隆

  单克隆

• 克隆编号

  BA-17

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab7755于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 3880 Human

• Omim: 148020 Human

• SwissProt: P08727 Human

• Unigene: 654568 Human

• 别名

• CK19 antibody

• Cytokeratin 19 antibody

• Cytokeratin-19 antibody

• K19 antibody

• K1C19_HUMAN antibody

• K1CS antibody

• Keratin 19 antibody

• Keratin type I 40 kD antibody

• Keratin type I 40kD antibody

• Keratin type I cytoskeletal 19 antibody

• Keratin, type I cytoskeletal 19 antibody

• Keratin, type I, 40 kd antibody

• Keratin-19 antibody

• KRT19 antibody

• MGC15366 antibody

• 40 kDa keratin intermediate filament antibody

• CK 19 antibody

• CK-19 antibody

• 

  Flow Cytometry (Intracellular) - Anti-Cytokeratin 19 antibody [BA-17] (ab7755)

  Separation of MCF-7 cells (red-filled) from human leukocytes (black-dashed) in flow cytometry analysis (intracellular staining) of peripheral whole blood spiked with MCF-7 cells stained using ab7755 (concentration in sample 3 μg/ml, GAM APC).

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 19 antibody [BA-17] (ab7755)

  ab7755 staining Cytokeratin 19 in wild-type MCF7 cells (top panel) and KRT19 knockout MCF7 cells (bottom panel). The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab7755 at 5µg/ml concentration and ab6046 (Rabbit polyclonal to beta Tubulin) at 1/1000 dilution overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to mouse IgG (Alexa Fluor® 488) (ab150117) at 2 μg/ml (shown in green) and a goat secondary antibody to rabbit IgG (Alexa Fluor® 594) (ab150080) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [BA-17] (ab7755)

  Human normal skin. Staining is observed in the cytoplasm (epidermal basal cells). Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control. Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for mouse for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

• 

  Western blot - Anti-Cytokeratin 19 antibody [BA-17] (ab7755)

  All lanes : Anti-Cytokeratin 19 antibody [BA-17] (ab7755) at 5 µg/ml
  
  Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
  Lane 2 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
  Lane 3 : MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
  
  Observed band size: 44 kDawhy is the actual band size different from the predicted?
  
  

• 

  Immunoprecipitation - Anti-Cytokeratin 19 antibody [BA-17] (ab7755)

  Cytokeratin 19 was immunoprecipitated using 0.5mg HepG2 whole cell extract, 5µg of Mouse monoclonal to Cytokeratin 19 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
  The antibody was incubated under agitation with Protein G beads for 10min, HepG2 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
  Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab7755.
  Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
  Band: 44kDa: Cytokeratin 19

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 19 antibody [BA-17] (ab7755)

  ICC/IF image of ab7755 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7755, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  
  

• 

  Western blot - Anti-Cytokeratin 19 antibody [BA-17] (ab7755)

  Anti-Cytokeratin 19 antibody [BA-17] (ab7755) + Cell lysates prepared from human MCF-7 cells
  

• 

  Flow Cytometry (Intracellular) - Anti-Cytokeratin 19 antibody [BA-17] (ab7755)

  Overlay histogram showing MCF7 cells stained with ab7755 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab7755, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF7 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.