Anti-Nestin抗体- Neural Stem Cell Marker
参阅全部 Nestin 一抗
兔多克隆抗体to Nestin
Rabbit
Detects a band at 200kDa representing Nestin in MEFs. Denaturing of WB protein lysate causes loss of Nestin immunoreactivity in WB.
适用于: WBmore details
不适用于: ICC/IF or IHC-P
与反应: Mouse
预测可用于: Rat, Rabbit, Hamster
Synthetic peptide:
ALETESQDSAEPSGSEE
, corresponding to amino acids 1481-1497 of Human Nestin.
ab7659 has been upgraded from Fast Track status based on data obtained in a new batch of this antibody.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride
浓度
100 µg 浓度为 1 mg/ml
100 µg 浓度为 1.1 mg/ml
Immunogen affinity purified
This affinity purified antibody is directed against human Nestin protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification.
多克隆
IgG
Abpromise™承诺保证使用ab7659于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) | 1/500 - 1/3000. Do not denature WB protein lysate, denaturing results in loss of Nestin specific immunoreactivity (See Abreview). |
应用说明
Is unsuitable for ICC/IF or IHC-P.
Entrez Gene: 18008 Mouse
SwissProt: Q6P5H2 Mouse
Unigene: 331129 Mouse
Unigene: 9701 Rat
Intermediate filament protein antibody
Nbla00170 antibody
nes antibody
NEST_HUMAN antibody
Nestin antibody
ESTM 46 antibody
FLJ 21841 antibody
FLJ21841 antibody
Western blot - Anti-Nestin antibody - Neural Stem Cell Marker (ab7659)
Anti-Nestin antibody - Neural Stem Cell Marker (ab7659) at 1/2000 dilution + MEF whole cell lysate at 30 µg
Secondary
IRDye 800
conjugated Goat anti-Rabbit IgG [H&L] at 1/10000 dilution
Performed under reducing conditions.
Observed band size: 200 kDawhy is the actual band size different from the predicted?
Western blot - Anti-Nestin antibody - Neural Stem Cell Marker (ab7659)This image is courtesy of Randal Moldrich, CNRS UMR7637, ESPCI, France
All lanes : Anti-Nestin antibody - Neural Stem Cell Marker (ab7659) at 1 µg/ml
Lane 1 : Mouse neural precursors whole cell lysate reduced but not denatured
Lane 2 : Mouse neural precursors whole cell lysate reduced and denatured
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 230 kDawhy is the actual band size different from the predicted?
Exposure time: 10 minutes
A slightly higher band obtained than expected, but as predicted in the datasheet. Denaturing of the WB lysate results in a loss of Nestin specific immunoreactivity. NB: Equal protein loading was verified.
