Anti-alpha Internexin antibody [2E3]

• 产品名称

  Anti-alpha Internexin抗体[2E3]
  参阅全部 alpha Internexin 一抗

• 描述

  小鼠单克隆抗体[2E3] to alpha Internexin

• 宿主

  Mouse

• 特异性

  specifically recognizes a-internexin.

• 经测试应用

  适用于: WB, Flow Cyt, ICC/IFmore details

• 种属反应性

  与反应: Mouse, Rat, Cow, Human
  预测可用于: Mammals

• 免疫原

  Recombinant full length protein corresponding to Rat alpha Internexin. (Purified from E. coli).

• 表位

  C-terminal non-helical extension of the protein.

• 阳性对照

• WB: Rat and mouse brain and spinal cord lysates; Cow spinal cord lysate. Flow Cyt: SH-SY5Y cells.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.03% Sodium azide
  Constituents: PBS, 50% Glycerol

• 纯度

  Tissue culture supernatant

• 克隆

  单克隆

• 克隆编号

  2E3

• 同种型

  IgG1

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab7654于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 9118 Human

• Entrez Gene: 226180 Mouse

• Entrez Gene: 24503 Rat

• Omim: 605338 Human

• SwissProt: Q16352 Human

• SwissProt: P46660 Mouse

• SwissProt: P23565 Rat

• Unigene: 500916 Human

• Unigene: 276251 Mouse

• Unigene: 88941 Rat

别名

• 66 kDa neurofilament protein antibody

• AINX_HUMAN antibody

• Alpha Inx antibody

• Alpha-internexin antibody

• Alpha-Inx antibody

• INA antibody

• Internexin neuronal intermediate filament protein alpha antibody

• MGC12702 antibody

• NEF 5 antibody

• NEF5 antibody

• Neurofilament 5 (66kD) antibody

• Neurofilament 5 antibody

• Neurofilament 66 antibody

• Neurofilament 66 tax binding protein antibody

• Neurofilament-66 antibody

• NF 66 antibody

• NF-66 antibody

• NF66 antibody

• TXBP 1 antibody

• TXBP1 antibody

• 

  Western blot - Anti-alpha Internexin antibody [2E3] (ab7654)

  All lanes : Anti-alpha Internexin antibody [2E3] (ab7654) at 1/10000 dilution
  
  Lane 1 : Rat brain lysate
  Lane 2 : Rat spinal cord lysate
  Lane 3 : Mouse brain lysate
  Lane 4 : Mouse spinal cord lysate
  Lane 5 : Cow spinal cord lysate
  
  Predicted band size: 66 kDa
  
  

• 

  Western blot - Anti-alpha Internexin antibody [2E3] (ab7654)

  All lanes : Anti-alpha Internexin antibody [2E3] (ab7654)
  
  Lanes 1-2 : Crude homogenate of rat facial nucleus at 3 days after experimental injury
  Lanes 3-4 : Crude homogenate of rat facial nucleus at 5 days after experimental injury
  Lanes 5-6 : Crude homogenate of rat facial nucleus at 7 days after experimental injury
  Lanes 7-8 : Crude homogenate of rat facial nucleus at 14 days after experimental injury
  Lanes 9-10 : Crude homogenate of rat facial nucleus at 28 days after experimental injury
  
  Predicted band size: 66 kDa
  Observed band size: 66 kDa
  
  

• 

  Flow Cytometry - Anti-alpha Internexin antibody [2E3] (ab7654)

  Overlay histogram showing SH-SY5Y cells stained with ab7654 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab7654, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.