Anti-IKKi/IKKe抗体
兔多克隆抗体to IKKi/IKKe
Rabbit
Recognises IKK epsilon and does not cross react with IKK alpha, beta or gamma.
适用于: ICC/IF, IHC-P, WBmore details
与反应: Mouse, Human
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS
浓度
100 µg 浓度为 1 mg/ml
Affinity purified
Purified IgG was prepared by affinity chromatography.
Nuclear factor kappa B (NF-kB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-kB is associated with IkB proteins in the cell cytoplasm, which inhibit NF-kB activity. IkB is phosphorylated by IkB kinase(IKK) complex that contains IKKa, IKKb, and IKKg. A novel molecule in the IKK complex was recently identified and designated IKKe/IKK-i. IKKe is required for the activation of NF-kB by PMA and T cell receptors but not by TNFa and IL-1. IKKe/IKK-i message is expressed in a variety of tissues and is inducible by TNFa, IL-1, and LPS.
多克隆
IgG
Abpromise™承诺保证使用ab7891于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | Use a concentration of 1 µg/ml. | |
| IHC-P | Use a concentration of 10 µg/ml. | |
| WB | (1) | Use a concentration of 1 µg/ml. Predicted molecular weight: 80 kDa. |
Entrez Gene: 9641 Human
Entrez Gene: 56489 Mouse
Omim: 605048 Human
SwissProt: Q14164 Human
SwissProt: Q9R0T8 Mouse
Unigene: 321045 Human
Unigene: 386783 Mouse
IKK related kinase epsilon antibody
IKK-E antibody
IKK-epsilon antibody
IKK-i antibody
IKKE antibody
IKKE_HUMAN antibody
IKKepsilon antibody
IKKI antibody
Inducible I kappa B kinase antibody
Inducible I kappa-B kinase antibody
Inducible IkappaB kinase antibody
Inhibitor of kappa light polypeptide gene enhancer in B cells kinase epsilon antibody
Inhibitor of kappa light polypeptide gene enhancer in B cells, kinase of, epsilon antibody
Inhibitor of nuclear factor kappa-B kinase subunit epsilon antibody
KIAA0151 antibody
MGC125294 antibody
MGC125295 antibody
MGC125297 antibody
I kappa B kinase epsilon antibody
I-kappa-B kinase epsilon antibody
IkBKE antibody
Western blot - Anti-IKKi/IKKe antibody (ab7891)
All lanes : Anti-IKKi/IKKe antibody (ab7891) at 1 µg/ml
Lane 1 : Untreated Raw264.7 cell lysates
Lane 2 : Raw264.7 cell lysates treated with LPS (0.3ug/ml) for 3 hrs
Lane 3 : Raw264.7 cell lysates treated with LPS (0.3ug/ml) for 6 hrs
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 80 kDa
Western blot - Anti-IKKi/IKKe antibody (ab7891)
All lanes : Anti-IKKi/IKKe antibody (ab7891) at 1 µg/ml
Lane 1 : A431 cell lysate
Lane 2 : Daudi cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : SK-N-SH cell lysate
Lane 5 : 3T3/NIH cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 80 kDa
Western blot - Anti-IKKi/IKKe antibody (ab7891)
Anti-IKKi/IKKe antibody (ab7891) at 1 µg/ml + Jurkat whole cell lysate
Predicted band size: 80 kDa
Observed band size: 80 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKKi/IKKe antibody (ab7891)
ab7891 at 10µg/ml staining IKK epsilon in human pancreatic tissue by IHC.
Immunocytochemistry/ Immunofluorescence - Anti-IKKi/IKKe antibody (ab7891)
ICC/IF image of ab7891 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7891, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunocytochemistry/ Immunofluorescence - Anti-IKKi/IKKe antibody (ab7891)
Immunocytochemistry/Immunofluorecense analysis of HeLa cells with ab7891 at 20µg/ml.
