重组蛋白G
参阅全部 Protein G 蛋白酶
> 95 % SDS-PAGE.
Chromatographically pure Protein G
Escherichia coli
Full length protein
No
Recombinant
Streptococcus
26 kDa
Our Abpromise guarantee covers the use of ab7458 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用
Conjugation
Lyophilized
Molecular Weight: 26.1 kDa. Approximately 5 mg of human IgG can be bound by 1 mg of Protein G. Optimal binding of Protein G to antibodies occurs at pH 5-6, with elution at pH 2.5-3. This product was expressed recombinantly in E. coli (non-native).
Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
复溶
Reconstitute with 1ml of distilled water to a concentration of 1mg/ml
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spg
spg immunoglobulin G-binding protein
SPG2_STRSG
IgG binding protein G
IgG-binding protein G
Immunoglobulin G binding protein G [Precursor]
Protein G is a bacterial protein derived from the cell wall of certain strains of b-hemolytic Streptococcci. It binds with high affinity to the Fc portion of various classes and subclasses of immunoglobulins from a variety of species. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse. Protein G binds preferentially to the Fc portion of IgG, but can also bind to the Fab region, making it useful for purification of F(ab') fragments of IgG. Due to it's affinity for the Fc region of many mammalian immunoglobulins, protein G is considered a universal reagent in biochemistry and immunology.
Cell Wall and Secreted
