Anti-Thymidylate Synthase antibody

• 产品名称

  Anti-Thymidylate Synthase抗体
  参阅全部 Thymidylate Synthase 一抗

• 描述

  羊多克隆抗体to Thymidylate Synthase

• 宿主

  Sheep

• 特异性

  In general, this antibody can detect antigen in a variety of human cells and tissues, as well as bacteria, African green monkey, rat and mouse. Somewhat lower dilutions may be required in some non-human cell lines. Anti-Thymidylate Synthase can detect Thymidylate Synthase by immunochemistry in proliferating cell cultures and tissues but does not stain nonproliferating cells. Normal colon mucosa shows weak staining; however, some colorectal cancer specimens show very strong staining.

• 经测试应用

  适用于: WBmore details

• 种属反应性

  与反应: Human
  不与反应: Saccharomyces cerevisiae

• 免疫原

  Recombinant full length protein corresponding to Human Thymidylate Synthase. Produced in E.coli.
  Database link: P04818

• 常规说明

  The antibody recognizes the expected additional band corresponding to the ternary complex of human TS-dFUMP-reduced folate in HeLa cells treated with the TS inhibitor 5-FUdR. This event occurs in most human breast, colorectal, gastric, head and neck carcinomas. The antibody recognizes 36 kDa human TS.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.01% Sodium azide
  Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab7398于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 7298 Human

• Omim: 188350 Human

• SwissProt: P04818 Human

• Unigene: 369762 Human

• 别名

• MGC88736 antibody

• OTTHUMP00000162195 antibody

• Thymidylate synthase antibody

• Thymidylate synthetase antibody

• TMS antibody

• TS antibody

• TSase antibody

• Tyms antibody

• TYMS protein antibody

• Tyms thymidylate synthetase antibody

• TYSY_HUMAN antibody

• d TMP synthase antibody

• EC 2.1.1.45 antibody

• HsT422 antibody

• 

  Western blot - Anti-Thymidylate Synthase antibody (ab7398)

  ab7398 is shown to detect thymidylate synthase present in a HeLa cell extract. Each lane is estimated to contain 4 µg of protein. Lanes 1, 2 and 3 represent 1/2000, 1/5000 and 1/10000 fold dilutions of the antibody. Detection was made using HRP Rabbit-a-Sheep IgG diluted 1/1000 and color development using TMB substrate for approximately 4'.