Anti-CD13 antibody [WM15]

• 产品名称

  Anti-CD13抗体[WM15]
  参阅全部 CD13 一抗

• 描述

  小鼠单克隆抗体[WM15] to CD13

• 宿主

  Mouse

• 经测试应用

  适用于: Flow Cyt, ICC/IFmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Tissue, cells or virus corresponding to Human CD13. Human AML cells

• 表位

  The antibody recognizes an extracellular epitope.

• 阳性对照

• ICC: THP-1, PANC-1 and A375 cells. Flow Cyt: Human peripheral blood leukocytes; Human Fibrosarcoma cells.

• 常规说明

  This product has switched fom a hybridoma to recombinant production method on 08th March 2021.

  This product is a recombinant monoclonal antibody, which offers several advantages including:

  For more information see here.

• - High batch-to-batch consistency and reproducibility

• - Improved sensitivity and specificity

• - Long-term security of supply

• - Animal-free production

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.40
  Preservative: 0.01% Sodium azide
  Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

• 纯度

  Protein A purified

• 纯化说明

  Purified by protein-A affinity chromatography > 95% pure (by SDS-PAGE).

• 克隆

  单克隆

• 克隆编号

  WM15

• 骨髓瘤

  NS1

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab7417于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 290 Human

• Omim: 151530 Human

• SwissProt: P15144 Human

• Unigene: 1239 Human

• 别名

• Aminopeptidase N antibody

• AMPN_HUMAN antibody

• ANPEP antibody

• AP M antibody

• AP N antibody

• AP-M antibody

• AP-N antibody

• APN antibody

• CD 13 antibody

• CD13 antibody

• CD13 antigen antibody

• gp150 antibody

• hAPN antibody

• LAP 1 antibody

• LAP1 antibody

• Microsomal aminopeptidase antibody

• Myeloid plasma membrane glycoprotein CD13 antibody

• p150 antibody

• PEPN antibody

• Alanyl (membrane) aminopeptidase antibody

• Alanyl aminopeptidase antibody

• Aminopeptidase M antibody

• 

  Immunocytochemistry/ Immunofluorescence - Anti-CD13 antibody [WM15] (ab7417)

  ab7417 staining CD13 in A375 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab7417 at 1µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor®; 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

  Also suitable in cells fixed with 4% paraformaldehyde (10 min).

  Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown

• 

  Flow Cytometry - Anti-CD13 antibody [WM15] (ab7417)

  Flow cytometry overlay histogram showing wild-type THP1 (green line) and ANPEP knockout THP1 cells (ab273759) stained with ab7417 (red line). The cells were incubated in 1x PBS containing 10μg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab7417) (1x106 in 100μl at 1 μg/ml) for 30 min at 4°C.

  The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor® 488, pre-adsorbed) (ab150117) was used at 1/2000 for 30 min at 4°C.

  Isotype control antibody was mouse IgG1κ (ab170190) used at the same concentration and conditions as the primary antibody (wild-type THP1 cells - black line; ANPEP knockout THP1 cells ab273759 - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).

  Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

  This image was generated from the hybridoma version of the product.

• 

  Immunocytochemistry - Anti-CD13 antibody [WM15] (ab7417)

  ab7417 staining CD13 in wild-type THP-1 cells (top panel) and ANPEP knockout THP-1 cells (bottom panel) (ab273759). The cells were fixed with 4% paraformaldehyde (10 min) then permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab7417 at 2.5μg/ml concentration and ab6046 (Rabbit polyclonal to beta Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to mouse IgG (Alexa Fluor® 488) (ab150117) at 2 μg/ml (shown in green) and a goat secondary antibody to rabbit IgG (Alexa Fluor® 594) (ab150080) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
  Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).

  This image was generated from the hybridoma version of the product.

• 

  Flow Cytometry - Anti-CD13 antibody [WM15] (ab7417)This image is courtesy of an anonymous Abreview

  This image was generated from the hybridoma version of the product.

  Ab7417 staining CD13 in Human Fibrosarcoma cells by flow cytometry. Cells were fixed with paraformaldehyde. The sample was incubated with primary antibody at 10µg/ml in PBS for 1 hour at 20°C. An Allophycocyanin (APC) conjugated anti-mouse monoclonal was used as a secondary antibody at 5µg/ml. Secondary antibody only (red). Ab7417 and secondary antibody (blue).

  See Abreview

• 

  Immunocytochemistry/ Immunofluorescence - Anti-CD13 antibody [WM15] (ab7417)

  ab7417 staining CD13 in PANC-1 cells (top panel - positive control) and HEK-293 cells (bottom panel - negative control). The cells were fixed with 4% paraformaldehyde (10 min) then permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab7417 at 0.5μg/ml concentration and ab6046 (Rabbit polyclonal to beta Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to mouse IgG (Alexa Fluor® 488) (ab150117) at 2 μg/ml (shown in green) and a goat secondary antibody to rabbit IgG (Alexa Fluor® 594) (ab150080) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
  Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).

• 

  Immunocytochemistry/ Immunofluorescence - Anti-CD13 antibody [WM15] (ab7417)This image is courtesy of an anonymous Abreview

  This image was generated from the hybridoma version of the product.

  Ab7417 staining CD13 in HT1080 cells by ICC/IF (Immunocytochemistry/Immunofluorescence). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at 10µg/ml for 1 hour. A Texas Red Goat Anti-mouse polyclonal was used as the secondary antibody.

  See Abreview