重组蛋白G (HRP)
参阅全部 Protein G 蛋白酶
> 98 % n/a.
Protein G Peroxidase was prepared from chromatographically pure recombinant Protein G.
Escherichia coli
Protein fragment
No
Recombinant
Streptococcus
L DKYGVSDYHK NLINNAKTVE GVKDLQAQVV ESAKKARISE ATDGLSDFLK SQTPAEDTVK SIELAEAKVL ANRELDKYGV SDYYKNLINN AKTVEGVKAL IDEILAALPK TDTYKLILNG KTLKGETTTE AVDAATAEKV FKQYANDNGV DGEWTYDDAT KTFTVTEKPE VIDASELTPA VTTYKLVING KTLK
63 kDa
190 to 384
HRP
Our Abpromise guarantee covers the use of ab7460 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用
Western blot
Dot blot
ELISA
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Lyophilized
No reaction was observed against anti-Protein A.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7.2
Preservative: 0.01% Gentamicin sulphate
Constituents: 1% BSA, 0.424% Potassium phosphate solution, 0.88% Sodium chloride
复溶
Reconstitute product with 1ml of deionized water.
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spg
spg immunoglobulin G-binding protein
SPG2_STRSG
IgG binding protein G
IgG-binding protein G
Immunoglobulin G binding protein G [Precursor]
Protein G is a bacterial protein derived from the cell wall of certain strains of b-hemolytic Streptococcci. It binds with high affinity to the Fc portion of various classes and subclasses of immunoglobulins from a variety of species. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse. Protein G binds preferentially to the Fc portion of IgG, but can also bind to the Fab region, making it useful for purification of F(ab') fragments of IgG. Due to it's affinity for the Fc region of many mammalian immunoglobulins, protein G is considered a universal reagent in biochemistry and immunology.
Cell Wall and Secreted
Western blot - Recombinant Protein G (HRP) (ab7460)Image courtesy of an anonymous Abreview.
Used for detection of primary antibody in WB after IP with the same antibody. In this case, IP was with goat anti HP1-alpha ab77256 on Protein G beads, and detection was with goat anti HP1-alpha ab77256, and Protein G HRP ab7460. Detection was very specific, and eliminated heavy and light chains of denatured antibodies.
Dot Blot - Recombinant Protein G (HRP) (ab7460)
Dot Blot analysis of ab7460 Protein G protein (HRP).
Load: 200ng of ab7460 followed by a 3-fold serial dilution
Antigens:
Row 1: Human IgG
Row 2: Rat IgG
Row 3: Dog IgG
Primary antibody: none
Secondary antibody: Protein G Peroxidase Conjugated at 1/1000 for 60 min at room temperature
Block: Blocking buffer for 60 min at room temperature
