Anti-CD31 antibody [MEC 7.46]

• 产品名称

  Anti-CD31抗体[MEC 7.46]
  参阅全部 CD31 一抗

• 描述

  大鼠单克隆抗体[MEC 7.46] to CD31

• 宿主

  Rat

• 特异性

  The antibody reacts with the murine form of the Platelet-Endothelial Cell Adhesion Molecule. The reactivity of the antibody is restricted to the isoform of the molecule that is selectively expressed by endothelial cells. The antibody precipitates a 130 kDa molecule present on the membrane of endothelial cell presents on all mouse blood vessels both in normal and inflamed or tumor tissues. The antigen is predominantly present at the lateral borders of endothelial cells as described for human PECAM-1.

• 经测试应用

  适用于: Flow Cyt, ICC/IFmore details
  不适用于: IP or WB

• 种属反应性

  与反应: Mouse
  

• 免疫原

  Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.

• 阳性对照

• ICC: mouse bEnd.3 cell line; Flow Cyt: mouse bEnd.3 cell line

• 常规说明

  This product has switched from a hybridoma to recombinant production method on 08th March 2021.

  This product is a recombinant monoclonal antibody, which offers several advantages including:

  For more information see here.

• - High batch-to-batch consistency and reproducibility

• - Improved sensitivity and specificity

• - Long-term security of supply

• - Animal-free production

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.01% Sodium azide
  Constituents: 59% PBS, 0.5% BSA, 40% Glycerol (glycerin, glycerine)

• 浓度

• 批次浓度范围 100 µg 浓度为 0.871 - 0.953 mg/ml

• 1 mg 浓度为 0.1 mg/ml

• 批次浓度范围 40 µg 浓度为 0.952 - 0.953 mg/ml

• 纯度

  Protein A purified

• 克隆

  单克隆

• 克隆编号

  MEC 7.46

• 同种型

  IgG1

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab7388于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用说明

Is unsuitable for IP or WB.

• 数据库链接

• Entrez Gene: 18613 Mouse

• SwissProt: Q08481 Mouse

• Unigene: 343951 Mouse

• 别名

• CD31 EndoCAM antibody

• EndoCAM antibody

• FLJ34100 antibody

• FLJ58394 antibody

• GPIIA antibody

• GPIIA' antibody

• PECA1 antibody

• PECA1_HUMAN antibody

• Pecam 1 antibody

• PECAM 1 CD31 EndoCAM antibody

• PECAM antibody

• PECAM-1 antibody

• Pecam1 antibody

• Platelet and endothelial cell adhesion molecule 1 antibody

• Platelet endothelial cell adhesion molecule antibody

• Platelet/endothelial cell adhesion molecule 1 antibody

• Adhesion molecule antibody

• CD31 antibody

• CD31 antigen antibody

• 

  Flow Cytometry - Anti-CD31 antibody [MEC 7.46] (ab7388)

  Flow cytometric analysis of / fixed / permeabilized B16-F0 (Mouse melanoma epithelial cell-like, Left) / bEnd.3 (Mouse brain endothelioma, Right) cells labelling CD31 with ab7388 at 1/1000 dilution (0.1µg) (Red) compared with a Rat monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, ab150161) at 1/2000 dilution was used as the secondary antibody. Negative control: B16-F0.Gated on viable cells.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-CD31 antibody [MEC 7.46] (ab7388)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized bEnd.3 cells labelling CD31 with ab7388 at 1/50 (19.32 µg/ml) dilution, followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in bEnd.3 cell line. Negative control: B16-F0 (PMID: 8082649) ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

  Secondary antibody only control: Secondary antibody is ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

• 

  Anti-CD31 antibody [MEC 7.46] (ab7388)