Anti-beta Synuclein抗体
参阅全部 beta Synuclein 一抗
兔多克隆抗体to beta Synuclein
Rabbit
Less than 0.1% cross reactivity against human alpha-synuclein Cross reactivity: This antibody is known to react with human and rat. Not yet tested in other species.
适用于: IHC-Pmore details
与反应: Human
Synthetic peptide (IEPLMEPEGSYEDPPQE) corresponding to the carboxyl terminal of human beta-synuclein (108- 125) and was conjugated to diphtheria toxoid via a cystine residue added to the carboxyl terminus of the peptide. The conjugate was cross-linked by maleimidocaproyl-N-hydroxy succinimide.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7
Whole antiserum
多克隆
IgG
Abpromise™承诺保证使用ab6165于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (1) | 1/500 - 1/2000. |
Entrez Gene: 6620 Human
Omim: 602569 Human
SwissProt: Q16143 Human
Unigene: 90297 Human
PNP14 antibody
Sncb antibody
Synuclein beta antibody
SYUB_HUMAN antibody
14 kDa brain-specific protein antibody
Beta-synuclein antibody
Phosphoneuroprotein 14 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Synuclein antibody (ab6165)
ab6165 staining beta synuclein in human occipital cortex.
Left panel: with primary antibody diluted 1:4000. Right panel: isotype control.
Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
