山羊F(ab')2抗兔IgG F(ab')2 (HRP)预吸附二抗
参阅全部 IgG 二抗
Goat
Rabbit
No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rabbit IgG F(c) or Bovine, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
适用于: ICC/IF, IHC-P, Dot blot, WB, ELISA, Electron Microscopy, IHCmore details
Cow, Horse, Human, Mouse, Rat, Sheepmore details
Rabbit IgG F(ab’)2 fragment
HRP
Liquid
Shipped at 4°C. Store at +4°C.
Preservative: 0.01% Gentamicin sulphate
Constituents: 1% BSA, 0.424% Potassium phosphate solution, 0.88% Sodium chloride
BSA is IgG and protease free
浓度
500 µg 浓度为 1 mg/ml
500 µg 浓度为 1 mg/ml
Affinity purified
Anti-Rabbit IgG F(ab')2 Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Horseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons)
多克隆
IgG
Abpromise™承诺保证使用ab6112于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | 1/1000 - 1/5000. | |
| IHC-P | 1/500. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. | |
| Dot blot | Use at an assay dependent concentration. | |
| WB | 1/2000 - 1/10000. | |
| ELISA | 1/100000. This product has been assayed against 1.0 ug of Rabbit IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/500 to 1/2000 of the reconstitution concentration is suggested for this product. Suitable for other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Read More | |
| Electron Microscopy | Use at an assay dependent concentration. | |
| IHC | Use at an assay dependent concentration. |
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat F(ab')2 Anti-Rabbit IgG F(ab')2 (HRP) preadsorbed (ab6112)
IHC image of beta Actin staining in normal human colon, formalin-fixed and paraffin-embedded tissue*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab8227, 3µg/ml overnight at +4°C. An HRP-conjugated secondary (Ab6112, 1/500 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.
The inset negative control image is taken from an identical assay without primary antibody.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
Western blot - Goat F(ab')2 Anti-Rabbit IgG F(ab')2 (HRP) preadsorbed (ab6112)
ab6112 was used at dilution 1/5000 with the primary antibody ab32117 in WB. See the review on ab32117.
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