Anti-Gli3抗体
参阅全部 Gli3 一抗
兔多克隆抗体to Gli3
Rabbit
A BLAST analysis was used to suggest cross reactivity with Gli-3 from human, chimpanzee, squirrel monkey, Xenopus laevis, chicken, dog and quail based on 100% sequence homology with the immunogen. Reactivity is also expected against homologues from mouse (94%) and rat (88%) based on partial homology. Reactivity with Gli-3 from other sources is not known.
适用于: ICC/IF, IHC-Fr, WB, IHC-Pmore details
与反应: Mouse, Human
预测可用于: Rat, Chicken, Xenopus laevis
Synthetic peptide:
NEDESPGQTYHRERRNA-C
conjugated to KLH, corresponding to amino acids 41-57 of Human GLI3.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride
浓度
100 µg 浓度为 1 mg/ml
100 µg 浓度为 1 mg/ml
Immunogen affinity purified
This affinity-purified antibody is directed against human Gli-3 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification.
多克隆
IgG
Abpromise™承诺保证使用ab6050于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (1) | Use a concentration of 1 µg/ml. See Abreview. |
| IHC-Fr | (1) | Use at an assay dependent concentration. |
| WB | Use at an assay dependent concentration. Predicted molecular weight: 170 kDa. PubMed: 23293081 | |
| IHC-P | Use a concentration of 0.5 - 5 µg/ml. |
Entrez Gene: 420769 Chicken
Entrez Gene: 2737 Human
Entrez Gene: 14634 Mouse
Omim: 165240 Human
SwissProt: Q9IA31 Chicken
SwissProt: P10071 Human
SwissProt: Q61602 Mouse
SwissProt: Q91660 Xenopus laevis
Unigene: 21509 Human
Unigene: 5098 Mouse
ACLS antibody
DNA binding protein antibody
GCPS antibody
Gli 3 antibody
GLI family zinc finger 3 antibody
GLI Kruppel family member GLI 3 antibody
GLI Kruppel family member GLI3 (Greig cephalopolysyndactyly syndrome) antibody
GLI Kruppel family member GLI3 antibody
GLI3 antibody
GLI3 C-terminally truncated form antibody
GLI3 form of 190 kDa antibody
GLI3 form of 83 kDa antibody
GLI3 full length protein antibody
GLI3-190 antibody
GLI3-83 antibody
GLI3_HUMAN antibody
GLI3FL antibody
Glioma associated oncogene family zinc finger 3 antibody
Oncogene GLI3 antibody
PAP A antibody
PAPA 1 antibody
PAPA antibody
PAPA1 antibody
PAPB antibody
PHS antibody
PPD IV antibody
PPDIV antibody
Transcriptional activator GLI3 antibody
Transcriptional repressor GLI3R antibody
Zinc finger protein GLI 3 antibody
Zinc finger protein GLI3 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gli3 antibody (ab6050)
ab6050 at 0.625 µg/ml staining human glioblastoma.
Immunocytochemistry/ Immunofluorescence - Anti-Gli3 antibody (ab6050)
ICC/IF image of ab6050 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6050, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
