Anti-4E-T抗体
参阅全部 4E-T 一抗
山羊多克隆抗体to 4E-T
Goat
适用于: IHC-P, WBmore details
与反应: Human
预测可用于: Mouse
Synthetic peptide corresponding to Human 4E-T aa 950 to the C-terminus (C terminal). (NP_001157974.1)
Database link: NP_062817.2
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Immunogen affinity purified
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
多克隆
IgG
Abpromise™承诺保证使用ab6034于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | Use a concentration of 5 µg/ml. | |
| WB | Use a concentration of 0.25 - 0.5 µg/ml. Detects a band of approximately 140-150 kDa. A 1 hour primary incubation is recommended for this product. The predicted molecular weight is 108 kDa according to NP_062817, however our observation agrees with that of Dostie et al, PMID: 10856257. Read More |
Entrez Gene: 56478 Human
Entrez Gene: 74203 Mouse
Omim: 607445 Human
SwissProt: Q9NRA8 Human
SwissProt: Q9EST3 Mouse
Unigene: 517559 Human
Unigene: 255649 Mouse
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Western blot - Anti-4E-T antibody (ab6034)
ab6034 at 0.25µg/ml staining approximately 140-150kDa band of 4E-T in HEK293 Lysate by Western blot (ECL). ab6034 at 0.25µg/ml staining approximately 140-150kDa band of EIF4ENIF1 in HEK293 Lysate by Western blot (ECL).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4E-T antibody (ab6034)
ab6034 at 5ug/ml staining 4E-T in human pancreas tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). The AP-staining procedure was used for detection.
