Anti-Tubulin antibody [YOL1/34] - Microtubule Marker

• 产品名称

  Anti-Tubulin抗体[YOL1/34] - Microtubule Marker
  参阅全部 Tubulin 一抗

• 描述

  大鼠单克隆抗体[YOL1/34] to Tubulin - Microtubule Marker

• 宿主

  Rat

• 经测试应用

  适用于: WB, ICC/IF, Flow Cyt (Intra)more details

• 种属反应性

  与反应: Mouse, Rat, Human
  预测可用于: Dog, Saccharomyces cerevisiae, Plants, Schizosaccharomyces pombe, a wide range of other species, Mammals, Alligator

• 免疫原

  Full length native protein (purified) corresponding to Saccharomyces cerevisiae Tubulin.

• 表位

  ab6161 binds to an epitope between amino acids 414 and 422 of alpha tubulin.

• 阳性对照

• WB: HeLa and NIH3T3 whole cell lysates and rat brain tissue lysate. Flow Cyt (Intra): methanol fixed/tween permeabilised HeLa cells. ICC/IF: HeLa, NIH/3T3 and human macrophage cells.

• 常规说明

  We can conjugate this antibody to FITC for you (please see ab150252 for details). This antibody can be used as a Western blotting loading control (Kops et al.) and as a Microtubule Marker.

  Has been used for the selection of specific recombinant antibodies engineered to incorporate its epitope. It is also useful for studying the function of microtubules.

  
  

  This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.40
  Preservative: 0.02% Sodium azide
  Constituents: PBS, 6.97% L-Arginine

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Protein G purified

• Primary antibody说明

  Has been used for the selection of specific recombinant antibodies engineered to incorporate its epitope. It is also useful for studying the function of microtubules.

• 克隆

  单克隆

• 克隆编号

  YOL1/34

• 同种型

  IgG2a

The Abpromise guarantee

Abpromise™承诺保证使用ab6161于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 10376 Human

• Entrez Gene: 203068 Human

• Entrez Gene: 7280 Human

• Entrez Gene: 22143 Mouse

• Entrez Gene: 22151 Mouse

• Entrez Gene: 22153 Mouse

• Entrez Gene: 500929 Rat

• Omim: 191130 Human

• Omim: 602530 Human

• Omim: 615101 Human

• SwissProt: P07437 Human

• SwissProt: P68363 Human

• SwissProt: Q13885 Human

• SwissProt: P05213 Mouse

• SwissProt: Q7TMM9 Mouse

• SwissProt: Q9D6F9 Mouse

• SwissProt: Q6P9V9 Rat

• Unigene: 524390 Human

• Unigene: 392113 Mouse

• Unigene: 99661 Rat

别名

• Tubulin beta 2b antibody

• Alpha tubulin antibody

• Alpha-tubulin ubiquitous antibody

• beta Ib tubulin antibody

• CDCBM5 antibody

• CDCBM6 antibody

• fd02b12 antibody

• K ALPHA 1 antibody

• M40 antibody

• OK/SW-cl.56 antibody

• TBA1B_HUMAN antibody

• TUBA1B antibody

• TUBB1 antibody

• TUBB2 antibody

• TUBB5 antibody

• tubulin alpha 1b antibody

• Tubulin alpha-1B chain antibody

• Tubulin alpha-ubiquitous chain antibody

• Tubulin beta 1b antibody

• Tubulin beta 2A antibody

• tubulin beta 2A class IIa antibody

• Tubulin beta antibody

• tubulin beta chain antibody

• tubulin beta class I antibody

• tubulin beta-1 chain antibody

• tubulin beta-2A chain antibody

• tubulin beta-5 chain antibody

• Tubulin K-alpha-1 antibody

• tubulin, alpha, ubiquitous antibody

• tubulin, beta 2A class IIa antibody

• tubulin, beta polypeptide 2 antibody

• tubulin, beta polypeptide antibody

• tubulin, beta, class IIA antibody

• wu:fd02b12 antibody

• zgc:55461 antibody

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)

  ab6161 staining Tubulin in HeLa cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab6161 at 5µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150165, Goat polyclonal Secondary Antibody to Rat IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

  Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

• 

  Western blot - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)

  Western blot against tubulin with ab6161 at 1/3000.  Secondary Rabbit anti-Rat IgG HRP (ab6734)was used at 1/2000.  Exposure time: 2mins.

  Lane 1: 20µg/lane HeLa (Human) whole cell lysates (ab7898).

  Lane 2: 20µg/lane 3T3 (Mouse) whole cell lysate (ab7901).

  Lane 3: 20µg/lane Rat brain tissue lysate (ab7942).

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)

  Confocal image of  21 day in vitro rat hippocampal neurons, stained with rat monoclonal antibody to Tubulin - Microtubule Marker (ab6161) in green at 1/500 and Microtubule Associated protein 2 in blue.

  This picture was kindly supplied as part of the review submitted by Dr Jonathon Burman.

• 

  Flow Cytometry (Intracellular) - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)

  Overlay histogram showing HeLa cells stained with ab6161 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab6161, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG2a [aRTK2758] (ab18450, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
  
  

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)

  Cultured human macrophages were used with ab6161 at 1/1000 for immunofluorescence. Cells were fixed with cold 2% formaldehyde for 20mins.

  Green staining is Alexa 568, Blue staining is DAPI stain.

  This cell represents a young macrophage, the staining patterns varied as the cells aged in culture.

• 

  Western blot - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)This image is courtesy of an anonymous Abreview

  All lanes : Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161) at 1/2000 dilution
  
  All lanes : Yeast (Saccharomyces cerevisiae) whole cell extract prepared by bead-beating
  
  Lysates/proteins at 5 µg per lane.
  
  Secondary
  All lanes : HRP conjugated goat anti-rat antibody
  
  Developed using the ECL technique.
  
  Performed under reducing conditions.
  
  Observed band size: 50 kDawhy is the actual band size different from the predicted?
  
  
  Exposure time: 30 seconds
  

  See Abreview

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)

  ICC/IF image of ab6161 stained human HepG2 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab6161, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa, HEK 293 and MCF7 cells.

• 

  Western blot - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)

  Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161) at 1 µg/ml + Brain (Rat) Tissue Lysate at 10 µg
  
  Secondary
  Rabbit polyclonal to Rat IgG - H&L (HRP) at 1/10000 dilution
  
  Developed using the ECL technique.
  
  Performed under reducing conditions.
  
  Observed band size: 54 kDawhy is the actual band size different from the predicted?
  
  
  Exposure time: 3 minutes
  

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)This image is courtesy of an anonymous Abreview

  ab6161 staining mouse NIH 3T3 fibroblast cells by ICC/IF. Cells were PFA fixed  and permeabilized in 0.2% Triton X-100 prior to blocking in 5% BSA for 45 minutes at RT.  The primary antibody was diluted 1/1000 and incubated with the sample for 1 hour.  An Alexa Fluor® 568 conjugated goat anti-rat antibody, diluted 1/3000, was used as the secondary.

   

  See Abreview

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)

  ab6161 staining tubulin HeLa cells treated with anisomycin (ab120495), by ICC/IF. Increase in tubulin expression correlates with increased concentration of anisomycin as described in literature.
  The cells were incubated at 37°C for 6h in media containing different concentrations of ab120495 (anisomycin) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab6161 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rat polyclonal antibody (ab98386) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.