Anti-PDE6 alpha抗体
参阅全部 PDE6 alpha 一抗
兔多克隆抗体to PDE6 alpha
Rabbit
适用于: IHC-P, WBmore details
与反应: Sheep, Human
预测可用于: Dog
Synthetic peptide corresponding to Human PDE6 alpha aa 1-16.
Sequence:
MGEVTAEEVEKFLDSN
(Peptide available as ab5862)
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab5659于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 90 kDa. representing PDE6 alpha from sheep retinal extract. A higher molecular weight band is sometimes observed using sheep retinal extracts. See Guo et al, Kerov et al for details of WB procedure. |
Entrez Gene: 5145 Human
Omim: 180071 Human
SwissProt: P16499 Human
Unigene: 567314 Human
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Western blot - Anti-PDE6 alpha antibody (ab5659)
Western blot of PDE6 alpha on sheep retinal extracts using ab5659.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE6 alpha antibody (ab5659)
IHC image of ab5659 staining in human renal carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab5659, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
