Anti-p38 (phospho T180 + Y182)抗体
参阅全部 p38 一抗
兔多克隆抗体to p38 (phospho T180 + Y182)
Rabbit
适用于: IHC-P, ICC/IF, WBmore details
与反应: Rat, Human
预测可用于: Mouse, Dog, Carp, Monkey
Synthetic peptide corresponding to Human p38 (phospho T180 + Y182). p38 is dually phosphorylated and therefore fully activated by MEK3 and MEK6 on threonine 180 and tyrosine 182 within the activation loop.
Database link: Q16539
(Peptide available as ab5253)
WB: HeLa, A431, COLO 205, A549 and A549 cell lysate; HEK-293 (human epithelial cell line from embryonic kidney) cells. IHC-P: Human brain tissue, human heart tissue, rat heart tissue. ICC: SH-SY5Y (human neuroblastoma cell line from bone marrow) cells.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA
BSA is IgG and protease free. PBS without Mg2+ and Ca2+.
浓度
50 µl 浓度为 0.2 mg/ml
Protein A purified
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using i) non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated p38, and ii) a JNK-derived peptide that is phosphorylated at threonine 183 and tyrosine 185. The final product is generated by affinity chromatography using a p38-derived peptide that is phosphorylated at threonine 180 and tyrosine 182.
多克隆
IgG
Abpromise™承诺保证使用ab4822于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | 1/10 - 1/100. | |
| ICC/IF | 1/250. | |
| WB | (3) | 1/1000. Predicted molecular weight: 38 kDa. |
Entrez Gene: 1432 Human
Entrez Gene: 26416 Mouse
Omim: 600289 Human
SwissProt: Q16539 Human
SwissProt: P47811 Mouse
Unigene: 485233 Human
Unigene: 311337 Mouse
Unigene: 88085 Rat
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Western blot - Anti-p38 (phospho T180 + Y182) antibody (ab4822)
All lanes : Anti-p38 (phospho T180 + Y182) antibody (ab4822) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) exposed for 40 min with UV, cell lysate
Lane 3 : A431 (human epidermoid carcinoma cell line) cell lysate
Lane 4 : A431 (human epidermoid carcinoma cell line) exposed for 40 min with UV, cell lysate
Lane 5 : COLO 205 (human colon adenocarcinoma cell line) cell lysate
Lane 6 : COLO 205 (human colon adenocarcinoma cell line) exposed for 40 min with UV, cell lysate
Lane 7 : A549 (human lung carcinoma cell line) cell lysate
Lane 8 : A549 (human lung carcinoma cell line) exposed for 40 min with UV, cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG HRP at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 38 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p38 (phospho T180 + Y182) antibody (ab4822)
Paraffin-embedded human brain tissue stained for p38 (phospho T180 + Y182) using ab4822 (right panel) at 1/100 dilution in immunohistochemical analysis followed by HRP-conjugated secondary antibody and DAB staining. Negative control (left panel) staining without primary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p38 (phospho T180 + Y182) antibody (ab4822)
Paraffin-embedded human heart tissue stained for p38 (phospho T180 + Y182) using ab4822 (right panel) at 1/20 dilution in immunohistochemical analysis followed by HRP-conjugated secondary antibody and DAB staining. Negative control (left panel) staining without primary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p38 (phospho T180 + Y182) antibody (ab4822)
Paraffin-embedded rat heart tissue stained for p38 (phospho T180 + Y182) using ab4822 (right panel) at 1/20 dilution in immunohistochemical analysis followed by HRP-conjugated secondary antibody and DAB staining. Negative control (left panel) staining without primary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-p38 (phospho T180 + Y182) antibody (ab4822)
4% PFA-fixed, Triton X-100 permeabilized SH-SY5Y (human neuroblastoma cell line from bone marrow) cells labeling p38 (phospho T180 + Y182) (Panel A: green) using ab4822 at 1 µg/mL in ICC/IF. Secondary antibody: Alexa Flour® 488 Goat Anti-Rabbit IgG at 1/400 dilution. Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 594 Phalloidin. Panel d is a merged image showing nuclear localization. Panel e is a no primary antibody control.
Western blot - Anti-p38 (phospho T180 + Y182) antibody (ab4822)
Peptide Competition: Extracts prepared from HEK-293 (human epithelial cell line from embryonic kidney) cells treated with UV irradiation were resolved on a 10% Tris-glycine gel and transferred to nitrocellulose. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4oC, then were incubated with 0.50 µg/mL ab4822 for two hours at room temperature in a 3% BSA-TBST buffer, following its prior incubation with: the peptide immunogen (1), a generic phosphothreonine containing peptide (2), a generic phosphotyrosine-containing peptide (3), the non-phosphorylated peptide corresponding to the phosphopeptide (4), no peptide (5), the phosphorylated peptide derived from the corresponding region of JNK 1 & 2 (6), and, the phosphorylated peptide derived from the corresponding region of ERK 1 & 2 (7). After washing, membranes were incubated with goat F(ab')2 antirabbit IgG alkaline phosphatase and the signal was detected using the Tropix WesternStar method. The data show that only the phosphopeptide
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