Anti-MKK7 (phospho S271 + T275)抗体
参阅全部 MKK7 一抗
兔多克隆抗体to MKK7 (phospho S271 + T275)
Rabbit
This antibody does not react with other MEK isoforms.
适用于: WBmore details
预测可用于: Mouse
Synthetic peptide corresponding to Human MKK7 (phospho S271 + T275).
WB: Fusion protein expressing MEK 7.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA
BSA is IgG and protease free
浓度
50 µl 浓度为 0.5 mg/ml
Immunogen affinity purified
Purified from rabbit serum by sequential epitope specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated MEK 7. The final product is generated by affinity chromatography using an MEK 7 derived peptide that is phosphorylated at serine 271 and threonine 275.
多克隆
IgG
Abpromise™承诺保证使用ab4762于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) | Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 38 kDa. |
Entrez Gene: 26400 Mouse
SwissProt: Q8CE90 Mouse
Unigene: 3906 Mouse
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Western blot - Anti-MKK7 (phospho S271 + T275) antibody (ab4762)
Peptide Competition: Extracts prepared from background extracts with tagged fusion protein expressing MEK 7 added were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4°C, then were incubated with 0.50 µg/mL ab4762 antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1), the non-phosphopeptide corresponding to the
