Anti-CYP2C9抗体
参阅全部 CYP2C9 一抗
兔多克隆抗体to CYP2C9
Rabbit
适用于: IHC-P, WBmore details
与反应: Mouse, Human
Recombinant full length protein corresponding to Human CYP2C9.
The Cytochrome P450 (P450) superfamily of enzymes is one of three enzyme systems which metabolize the fatty acid arachadonic acid (AA) to regulators of vascular tone. P450 enzymes are monooxygenase enzymes which require several co-factors such as nicotinamide adenine dinucleotide phosphate (NADPH) and P450 reductase. There are over 200 known genes which encode P450s. Epoxygenases are those P450s which metabolize AA to epoxyeicosatrienoic acid (EETs) and omega-hydroxylases are those P450s which produce 19- and 20-hydroxyeicosatetraenoic acids (19- and 20-HETE). As well as fatty acid metabolism, P450s also metabolize many drugs and toxins. Cytochrome P450 3A4 is abundantly expressed in liver and small intestine and is inducible by barbiturates, glucocorticoids and rifampicin.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 8.00
Constituent: 0.0536% PBS
浓度
100 µl 浓度为 1 mg/ml
Ammonium Sulphate Precipitation
The Cytochrome P450 (P450) superfamily of enzymes is one of three enzyme systems which metabolize the fatty acid arachadonic acid (AA) to regulators of vascular tone. P450 enzymes are monooxygenase enzymes which require several co-factors such as nicotinamide adenine dinucleotide phosphate (NADPH) and P450 reductase. There are over 200 known genes which encode P450s. Epoxygenases are those P450s which metabolize AA to epoxyeicosatrienoic acid (EETs) and omega-hydroxylases are those P450s which produce 19- and 20-hydroxyeicosatetraenoic acids (19- and 20-HETE). As well as fatty acid metabolism, P450s also metabolize many drugs and toxins. Cytochrome P450 3A4 is abundantly expressed in liver and small intestine and is inducible by barbiturates, glucocorticoids and rifampicin.
多克隆
IgG
Abpromise™承诺保证使用ab4236于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | (2) | Use at an assay dependent concentration. Predicted molecular weight: 56 kDa. Positive control 0.1 µg of recombinant human cytochrome P450 2. |
Entrez Gene: 1559 Human
Omim: 601130 Human
SwissProt: P11712 Human
Unigene: 282624 Human
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Cytochrome P-450MP antibody
Cytochrome P450 2C9 antibody
Cytochrome P450 MP-4 antibody
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Cytochrome P450 PB-1 antibody
Cytochrome P450, family 2, subfamily C, polypeptide 9 antibody
Cytochrome p4502C9 antibody
flavoprotein-linked monooxygenase antibody
MGC149605 antibody
MGC88320 antibody
microsomal monooxygenase antibody
OTTHUMP00000020135 antibody
P450 MP antibody
P450 PB 1 antibody
P450 PB1 antibody
P450IIC9 antibody
P450MP antibody
S mephenytoin 4 hydroxylase antibody
S-mephenytoin 4-hydroxylase antibody
xenobiotic monooxygenase antibody
(R)-limonene 6-monooxygenase antibody
(S)-limonene 6-monooxygenase antibody
(S)-limonene 7-monooxygenase antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP2C9 antibody (ab4236)
IHC image of ab4236 staining in human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab4236, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
