Anti-IRS1 (phospho Y896)抗体
参阅全部 IRS1 一抗
兔多克隆抗体to IRS1 (phospho Y896)
Rabbit
适用于: IHC-Pmore details
与反应: Human
预测可用于: Mouse, Rat
Synthetic peptide corresponding to IRS1 (phospho Y896).
Chinese Hamster Ovary cell line transfected with a vector encoding the human insulin receptor (CHO-T) and transiently transfected with a vector encoding IRS 1 and stimulated with insulin.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.3
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA
BSA is IgG and protease free
浓度
50 µl 浓度为 0.4 mg/ml
Immunogen affinity purified
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated insulin receptor substrate 1 (IRS 1).
多克隆
IgG
Abpromise™承诺保证使用ab4873于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | 1/10 - 1/100. |
Entrez Gene: 3667 Human
Entrez Gene: 16367 Mouse
Omim: 147545 Human
SwissProt: P35568 Human
SwissProt: P35569 Mouse
Unigene: 471508 Human
Unigene: 4952 Mouse
Unigene: 10476 Rat
HIRS 1 antibody
HIRS1 antibody
Insulin receptor substrate 1 antibody
IRS 1 antibody
IRS-1 antibody
IRS1 antibody
IRS1_HUMAN antibody
OTTHUMP00000164234 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRS1 (phospho Y896) antibody (ab4873)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling IRS1 with ab4873 at 1/20 dilution. Antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature and washed with ddH2O and PBS. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
