Anti-ILF1抗体
参阅全部 ILF1 一抗
山羊多克隆抗体to ILF1
Goat
适用于: Flow Cyt (Intra), ICC/IF, WBmore details
与反应: Human
Synthetic peptide: TPPAAVREKGVQN, corresponding to C terminal amino acids 648-660 of Human ILF1.
Flow Cyt (Intra): HeLa cells.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA
Immunogen affinity purified
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
多克隆
IgG
Abpromise™承诺保证使用ab5298于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) | Use a concentration of 10 µg/ml. | |
| ICC/IF | Use a concentration of 10 µg/ml. | |
| WB | Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 75-80 kDa (predicted molecular weight: 73 kDa). A 1 hour primary incubation is recommended for this product. |
Entrez Gene: 3607 Human
Omim: 147685 Human
SwissProt: Q01167 Human
Unigene: 591140 Human
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Immunocytochemistry/ Immunofluorescence - Anti-ILF1 antibody (ab5298)
Immunofluorescent analysis of paraformaldehyde fixed HeLa cells, labeling ILF-1 with ab5298. Cells permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nuclear and cytoplasmic/vesicle staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
Flow Cytometry (Intracellular) - Anti-ILF1 antibody (ab5298)
Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line) labeling ILF1 with ab5298. Cells permeabilized with 0.5% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Western blot - Anti-ILF1 antibody (ab5298)
All lanes : Anti-ILF1 antibody (ab5298) at 0.03 µg/ml
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) nuclear cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) nuclear cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) nuclear cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 73 kDa
Observed band size: 80 kDawhy is the actual band size different from the predicted?
Immunocytochemistry/ Immunofluorescence - Anti-ILF1 antibody (ab5298)
Immunofluorescent analysis of paraformaldehyde fixed U2OS cells, labeling ILF-1 with ab5298. Cells permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nuclear and cytoplasmic/vesicle staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
Immunocytochemistry/ Immunofluorescence - Anti-ILF1 antibody (ab5298)Image from Marais A et al., J Biol Chem. 2010 Nov 12;285(46):35728-39. Epub 2010 Sep 1. Fig 2.; doi: 10.1074/jbc.M110.154005; November 12, 2010, The Journal of Biological Chemistry, 285, 35728-35739.
Immunofluorescence analysis of U2OS cells, staining ILF1 with ab5298. Cells were fixed with 4% paraformaldehyde and incubated with primary antibody at 1/100 dilution. A FITC-conjugated rabbit anti-goat IgG was used as the secondary antibody.
