Anti-GFP antibody

• 产品名称

  Anti-GFP抗体
  参阅全部 GFP 一抗

• 描述

  山羊多克隆抗体to GFP

• 宿主

  Goat

• 特异性

  Reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP and EGFP.

• 经测试应用

  适用于: WB, IPmore details

• 种属反应性

  与反应: Species independent
  

• 免疫原

  Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

• 阳性对照

• Pure GFP protein, or cells known to overexpress GFP.

• 常规说明

  Protein A will not bind goat IgG, so use alternates (eg. protein G) in IP with this antibody. This antibody is available in an affinity purified form as ab5450.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

• 存储溶液

  Preservative: 0.05% Sodium azide

• 纯度

  Whole antiserum

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab5449于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 别名

• GFP antibody

• Green fluorescent protein antibody

• 

  Western blot - Anti-GFP antibody (ab5449)

  Lane 1       :  parental YTS cells (negative control)
  Lanes 2-5 :  YTS cells transfected with KIR-EGFP (mw 88 kD)

  KIR-EGFP IP's with Goat polyclonal to GFP (ab5449) using 0.1 ul for 2x106 cells. KIR-EGFP detected with a mouse monoclonal to KIR receptor (Borszcz et al EGI 2003, 33: 1084).

   

  
  
  Lane 1 : parental YTS cells (negative control) Lanes 2-5 : YTS cells transfected with KIR-EGFP (mw 88 kD) KIR-EGFP IP's with Goat polyclonal to GFP (ab5449) using 0.1 ul for 2x106 cells. KIR-EGFP detected with a mouse monoclonal to KIR receptor (Borszcz et al EGI 2003, 33: 1084).

• 

  Western blot - Anti-GFP antibody (ab5449)

  5 ng GFP on  PVDF membrane QC. Goat polyclonal to GFP (ab5449) used at dilutions of:
  Lane 1 : 1/2500
  Lane 2 : 1/5000
  Lane 3 :  1/10,000
  Lane 4 :  1/20,000

  
  
  5 ng GFP on PVDF membrane QC. Goat polyclonal to GFP (ab5449) used at dilutions of: Lane 1 : 1/2500 Lane 2 : 1/5000 Lane 3 : 1/10,000 Lane 4 : 1/20,000