Anti-Aurora B抗体
参阅全部 Aurora B 一抗
兔多克隆抗体to Aurora B
Rabbit
适用于: ICC/IF, IHC-P, WBmore details
与反应: Mouse, Rat, Human
预测可用于: Hamster, Pig
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab2254于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (13) | Use a concentration of 0.5 - 1 µg/ml. Methanol fixation recommended. |
| IHC-P | (4) | 1/200. |
| WB | (14) | 1/1000 - 1/2000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa). |
Entrez Gene: 9212 Human
Entrez Gene: 20877 Mouse
Omim: 604970 Human
SwissProt: Q96GD4 Human
SwissProt: O70126 Mouse
Unigene: 442658 Human
Unigene: 3488 Mouse
Unigene: 10865 Rat
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Immunocytochemistry/ Immunofluorescence - Anti-Aurora B antibody (ab2254)
ab2254 stained in Hela cells. Cells were fixed with 4% paraformaldehyde (10min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% triton for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab2254 at 1µg/ml and ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control) at 1/1000 dilution overnight at +4°C. The secondary antibodies were ab150120 (pseudo-colored red) and ab150081 (colored green) used at 1 ug/ml for 1hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43µM for 1hour at room temperature.
Western blot - Anti-Aurora B antibody (ab2254)Balboula and Schindler PLoS Genet. 2014 Feb 27;10(2):e1004194. doi: 10.1371/journal.pgen.1004194. eCollection 2014 Feb. Fig 1. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
AURKB is expressed in mouse oocytes.
(Panel D) 20 GV-intact oocytes were collected from CF1 mice and micro-injected with the indicated cRNA. Two hours after injection, the oocytes were matured to Met II in vitro (16 h). The total numbers of non-injected control oocytes (Non-inj.) are indicated in parenthesis. Total cellular lysates were probed with the indicated antibody. The panels are images of the same membrane that was stripped and re-probed. The arrows indicate the specific AURKB protein band, and the asterisk indicates a presumed degradation product of AURKB-GFP.
Immunocytochemistry/ Immunofluorescence - Anti-Aurora B antibody (ab2254)Balboula and Schindler PLoS Genet. 2014 Feb 27;10(2):e1004194. doi: 10.1371/journal.pgen.1004194. eCollection 2014 Feb. Fig 1.
AURKB is expressed in mouse oocytes.
(Panel A) GV-intact oocytes were collected from CF1 mice and matured in vitro for 8 h (Met I), or 16 h (Met II), prior to fixation and staining with an anti-AURKB antibody (ab2254).
Immunocytochemistry/ Immunofluorescence - Anti-Aurora B antibody (ab2254)Chopra et al PLoS One. 2016 Apr 20;11(4):e0153818. doi: 10.1371/journal.pone.0153818. eCollection 2016. Fig 6. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
HCT 116 (Human colorectal carcinoma cell line) cells were examined by immunofluorescence confocal microscopy.
Cells were treated with 500 nM AK301 for 16 hours, and then processed for Aurora B (ab2254) and β-tubulin staining (Panel B). The color key and 20 μm bars are shown. Images of representative field is shown with a 20 μm bar. End-labeled DNA is shown in red and DAPI-stained DNA is blue.
Cells cultured on coverslips were fixed with 4% paraformaldehyde at room temperature or 100% ice cold methanol at 4°C and then permeabilized with 0.5% Triton X-100 in PBS. Cells were blocked in 5% serum (in PBS) and then incubated with primary antibody (in 5% serum) on shaker for 1 h at room temperature.
Western blot - Anti-Aurora B antibody (ab2254)
All lanes : Anti-Aurora B antibody (ab2254) at 1 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : HeLa nocodozole treated cell lysate
Lane 3 : NIH3T3 cell lysate
Lane 4 : PC12 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Performed under reducing conditions.
Predicted band size: 39 kDa
Blocked with 2% BSA.
Immunocytochemistry/ Immunofluorescence - Anti-Aurora B antibody (ab2254)
Immunofluorescence in human cells using Rabbit polyclonal to Aurora B (red), DAPI (blue) and CREST serum (binds to centromeres)(green).
(a) HeLa cells - transition from interphase (left) through mitosis
(b) RPE-1 cells - as in (a)
(c) HeLa cells - interphase
(d) RPE-1 cells - interphase
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aurora B antibody (ab2254)
IHC image of Aurora B staining in Human Lymph node Hodgkins disease formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2254, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
Immunocytochemistry/ Immunofluorescence - Anti-Aurora B antibody (ab2254)This image is courtesy of an Abreview from Lux Fatimathas.
ab2254 staining human A431 (epithelial) cells by ICC/IF. The sample was fixed in paraformaldehyde and permeabilized by incubation with 0.1% Triton X100. 1% BSA was used as the blocking agent prior to a 1 hour incubation with the primary antibody, diluted 1/1000 with 1% BSA made up in PBS. An Alexa Fluor® 647 conjugated Donkey anti-Rabbit IgG (H+L) antibody was used as the secondary. Blocking and antibody incubation steps were carried out at room temperature.
In this set of images, the tubulin is stained green, Aurora B in pink and DNA in blue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aurora B antibody (ab2254)
Rabbit polyclonal to Aurora B (ab2254) used to stain SW620 human tumour xenografts (in mouse).
The sections were microwave pretreated in citrate buffer (pH 6.0) for 5 mins high then 5 mins simmer (800W conventional microwave). Slides were then incubated for 1 hour with the Aurora B primary antibody diluted 1/200 in TBS, then visualised using DAB, after application of an appropriate secondary.
Western blot - Anti-Aurora B antibody (ab2254)
All lanes : Anti-Aurora B antibody (ab2254) at 1 µg/ml
Lane 1 : HeLa Whole Cell Lysate
Lane 2 : HeLa Nuclear Lysate
Lane 3 : Jurkat Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 39 kDa
Observed band size: 39 kDa
Additional bands at: 37 kDa (possible isoform)
Exposure time: 150 seconds
Western blot - Anti-Aurora B antibody (ab2254)
Anti-Aurora B antibody (ab2254) at 1/2000 dilution + Recombinant human Aurora B protein (ab51435) at 0.1 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 39 kDa
