抗人Serum Albumin抗体
参阅全部 Human Serum Albumin 一抗
兔多克隆抗体to人Serum Albumin
Rabbit
适用于: Sandwich ELISA, IHC-Pmore details
与反应: Human
Full length native protein (purified) corresponding to Human Serum Albumin.
IHC-P: Human liver tissue.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.3
Preservative: 0.05% Sodium azide
Constituent: 1% BSA
浓度
Protein A purified
多克隆
IgG
unknown
Abpromise™承诺保证使用ab2406于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [15C7] to Human Serum Albumin (ab10241). For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [15C7] to Human Serum Albumin (ab10241) as Capture. Read More | |
| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Entrez Gene: 213 Human
Omim: 103600 Human
SwissProt: P02768 Human
Unigene: 418167 Human
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Serum Albumin antibody (ab2406)
Formalin-fixed, paraffin-embeded human liver tissue stained for Human Serum Albumin using ab2406 at 1/50 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Serum Albumin antibody (ab2406)
Ab2406 staining human normal liver. Staining is localised to the cytoplasm and secreted.
Left panel: with primary antibody. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffers citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be re
Sandwich ELISA - Anti-Human Serum Albumin antibody (ab2406)
Standard Curve for Human Serum Albumin (Analyte: Human Serum Albumin protein (ab67670)); using Capture Antibody Mouse monoclonal [15C7] to Human Serum Albumin (ab10241) at 1µg/ml and Detector Antibody Rabbit polyclonal to Human Serum Albumin (ab2406) .
