Anti-MAP2抗体
参阅全部 MAP2 一抗
鸡多克隆抗体to MAP2
Chicken
适用于: ICC/IF, IHC-P, WBmore details
与反应: Mouse, Rat
Recombinant fragment corresponding to Human MAP2. Mix of recombinant human constructs of projection domain sequences, amino acids 235-1588.
IHC-P: Rat cerebellum tissue. ICC: Rat E20 cultured cortical neuron-glial cells, rat cortical neurons, rat cortical neurons and glia in mixed tissue culture and mouse neurons. WB: Adult rat brain, Embryonic E20 rat brain and Adult mouse brain lysates.
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Liquid
Shipped at 4°C. Store at +4°C. Do Not Freeze.
Preservative: 0.032% Sodium azide
Constituent: PBS
Lipid extraction from egg yolk, followed by ammonium sulphate precipitation.
多克隆
IgY
Abpromise™承诺保证使用ab5392于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (29) | 1/5000 - 1/10000. |
| IHC-P | (13) | 1/10000. |
| WB | (6) | 1/20000 - 1/100000. This 280kDa band corresponds to the high molecular weight MAP2a and MAP2b isoforms. Also detects 2 bands at approximately 70 kDa, corresponding to MAPc, since the 70kDa bands are transcripts from the same gene and correspond to the C-terminus of the 280kDa bands. Read More |
Entrez Gene: 17756 Mouse
SwissProt: P20357 Mouse
Unigene: 256966 Mouse
Unigene: 436793 Mouse
Unigene: 10484 Rat
Unigene: 136301 Rat
Unigene: 230443 Rat
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody (ab5392)
Chromogenic Immunostaining of a formalin fixed paraffin embedded rat cerebellum section with chicken pAb to MAP2A/B dilution 1/10000, detected in DAB (brown) following the ABC method. Hematoxylin (blue) was used as the counterstain. ab5392 detects neuronal cells, specifically their perikarya and dendrites. Note the prominent staining of Purkinje cells and their dendrites in the molecular layer and staining of the much smaller granule neurons in the granular layer.
Immunocytochemistry - Anti-MAP2 antibody (ab5392)
Rat E20 cultured cortical neuron-glial cells stained for MAP2 (red) using ab5392 at 1/10000 dilution for ICC/IF. Tau is detected with a mouse monoclonal anti-Tau antibody (green). The nuclear counter stain is DAPI (blue). Overlap of MAP2 and Tau staining results in an orange-yellow color.
Western blot - Anti-MAP2 antibody (ab5392)
All lanes : Anti-MAP2 antibody (ab5392) at 1/50000 dilution
Lane 1 : Adult rat brain lysate
Lane 2 : Embryonic E20 rat brain lysate
Lane 3 : Adult mouse brain lysate
Immunocytochemistry - Anti-MAP2 antibody (ab5392)
ab5392 at a dilution of 1/10000, staining MAP 2 (green) in tissue cultured rat cortical neurons by immunofluorescence. Nuclei are stained blue with Dapi.
Immunocytochemistry - Anti-MAP2 antibody (ab5392)
Rat cortical neurons and glia in mixed tissue culture stained with ab5392 (Chicken antibody to MAP2) (green)(1:30 000), a mouse monoclonal antibody to GFAP (red) and nuclei of all cells stained with Hoechst dye (blue).
Immunocytochemistry - Anti-MAP2 antibody (ab5392)This image is courtesy of an anonymous Abreview
ab5392 staining MAP2 in mouse neurons by immunocytochemistry/ immunofluorescence. Cells were PFA fixed and permeabilized in 0.4% Triton X-100 prior to blocking in 5% serum for 1 hour at 25°C. The primary antibody was diluted 1/10000 and incubated with the sample for 20 hours at 21°C. Alexa fluor® 488 goat polyclonal to chicken IgY, diluted 1/400, was used as the secondary antibody.
Western blot - Anti-MAP2 antibody (ab5392)This image is courtesy of an anonymous Abreview
Anti-MAP2 antibody (ab5392) at 1/10000 dilution + Mouse brain lysate - post nuclear supernatant at 10 µg
Secondary
HRP-conjugated rabbit anti-chicken IgY at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 280,70 kDawhy is the actual band size different from the predicted?
Exposure time: 1 minute
