Anti-PYK2 (phospho Y579 + Y580)抗体
参阅全部 PYK2 一抗
兔多克隆抗体to PYK2 (phospho Y579 + Y580)
Rabbit
Some cross-reactivity still may be experienced in cases where Focal Adhesion Kinase is overexpressed relative to PYK 2 within the same cell system.
适用于: IHC-P, ICCmore details
与反应: Mouse, Human
Synthetic peptide corresponding to Human PYK2 (phospho Y579 + Y580). The sequence is conserved in mouse and rat.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.3
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
Immunogen affinity purified
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using (i) a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated PYK 2, (ii) a generic tyrosine phosphorylated peptide to remove antibody that is reactive with phosphotyrosine, irrespective of the sequence, and (iii) a phosphopeptide derived from the corresponding region of Focal Adhesion Kinase (a PYK 2-related protein) to remove antibody that is reactive with phosphorylated Focal Adhesion Kinase protein. The final product is generated by affinity chromatography using a PYK 2-derived peptide that is phosphorylated at tyrosines 579 and 580.
多克隆
IgG
Abpromise™承诺保证使用ab4807于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | 1/10 - 1/100. | |
| ICC | 1/250. |
Entrez Gene: 2185 Human
Entrez Gene: 19229 Mouse
Omim: 601212 Human
SwissProt: Q14289 Human
SwissProt: Q9QVP9 Mouse
Unigene: 491322 Human
Unigene: 21613 Mouse
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Immunocytochemistry - Anti-PYK2 (phospho Y579 + Y580) antibody (ab4807)
Immunocytochemistry analysis of A549 cells labelling PYK2 with ab4807 at 1/250 dilution. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. A Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate was used as a secondary antibody at 1:2000. Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin. Panel d is a merged image showing membranous localization. Panel e is untreated cell with no signal. Panel f is a no primary antibody control. The images were captured at 60X magnification.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYK2 (phospho Y579 + Y580) antibody (ab4807)
Immunohistochemistry analysis of human spleen tissue labelling Phospho-Pyk2 (pYpY579/580) with ab4807 at 1/20 dilution. Antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting. A no was also produced antibody control (left).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYK2 (phospho Y579 + Y580) antibody (ab4807)
Immunohistochemistry analysis of mouse brain tissue labelling Phospho-Pyk2 (pYpY579/580) with ab4807 at 1/20 dilution. Antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting. A no was also produced antibody control (left).
