Anti-Cleaved Caspase-3抗体
参阅全部 Cleaved Caspase-3 一抗
兔多克隆抗体to Cleaved Caspase-3
Rabbit
适用于: WBmore details
与反应: Human, Recombinant fragment
Synthetic peptide corresponding to Human Cleaved Caspase-3. Synthetic peptide mapping to the N-terminus adjacent to the cleavage site of human caspase-3
Database link: P42574
(Peptide available as ab38283)
WB: HeLa (treated with staurosporine) whole cell lysate. Camptothecin treated Jurkat whole cell lysate. Cleaved Caspase 3 recombinant protein.
This product is manufactured by BioVision, an Abcam company and was previously called 3015 Anti-Caspase-3 (Active) Polyclonal Antibody.
Caspases are synthesized as inactive pro-enzymes that are processed to active form in cells undergoing apoptosis. Caspase 3 has been extensively studied and implicated to play an important role in apoptosis. Active caspase 3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells (e.g., PARP). This affinity purified antibody recognizing the active forms of caspase-3 provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells.
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Liquid
Shipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Preservative: 0.03% Proclin 300
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 0.5% BSA, 0.146% EDTA
浓度
50 µg 浓度为 0.5 mg/ml
50 µg 浓度为 0.5 mg/ml
Immunogen affinity purified
Caspases are synthesized as inactive pro-enzymes that are processed to active form in cells undergoing apoptosis. Caspase 3 has been extensively studied and implicated to play an important role in apoptosis. Active caspase 3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells (e.g., PARP). This affinity purified antibody recognizing the active forms of caspase-3 provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells.
多克隆
IgG
Abpromise™承诺保证使用ab2302于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (15) | 1/50 - 1/500. Detects a band of approximately 17 kDa. |
Entrez Gene: 836 Human
Omim: 600636 Human
SwissProt: P42574 Human
Unigene: 141125 Human
CASP-3 antibody
CASP3 antibody
CASP3_HUMAN antibody
Caspase 3 antibody
Caspase-3 subunit p12 antibody
CPP 32 antibody
CPP-32 antibody
CPP32B antibody
Cysteine protease CPP32 antibody
PARP cleavage protease antibody
Protein Yama antibody
SCA-1 antibody
SCA1 antibody
SREBP cleavage activity 1 antibody
Yama antibody
active caspase 3 antibody
Apopain antibody
CASP 3 antibody
Western blot - Anti-Cleaved Caspase-3 antibody (ab2302)This image is courtesy of an anonymous Abreview
Anti-Cleaved Caspase-3 antibody (ab2302) at 1/2000 dilution + Poly I:C treated Melanoma cells at 20 µg
Secondary
Goat anti-rabbit IgG (HRP) at 1/1000 dilution
Developed using the ECL technique.
Observed band size: 17 kDawhy is the actual band size different from the predicted?
Exposure time: 20 seconds
Western blot - Anti-Cleaved Caspase-3 antibody (ab2302)
All lanes : Anti-Cleaved Caspase-3 antibody (ab2302)
Lane 1 : Untreated Jurkat cell line (human T cell leukemia cell line from peripheral blood)
Lane 2 : Camptothecin treated Jurkat cell line (human T cell leukemia cell line from peripheral blood)
Lysates/proteins at 60 µg per lane.
Western blot - Anti-Cleaved Caspase-3 antibody (ab2302)
All lanes : Anti-Cleaved Caspase-3 antibody (ab2302) at 1 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (2 uM Staurosporine, 4Hr) at 20 µg
Lane 2 : HeLa whole cell lysate (untreated) at 20 µg
Lane 3 : Cleaved Caspase 3 (recombinant protein) at 0.1 µg
Secondary
All lanes : 800CW Goat Anti-Rabbit IgG at 1/10000 dilution
Performed under reducing conditions.
Additional bands at: 17 kDa (possible mature (processed) protein), 24 kDa, 35 kDa. We are unsure as to the identity of these extra bands.
Western blot - Anti-Cleaved Caspase-3 antibody (ab2302)
Anti-Cleaved Caspase-3 antibody (ab2302) at 1 µg/ml + Camptothecin
(2 µM) treated Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
