Anti-TRIM21/SS-A antibody

• 产品名称

  Anti-TRIM21/SS-A抗体
  参阅全部 TRIM21/SS-A 一抗

• 描述

  山羊多克隆抗体to TRIM21/SS-A

• 宿主

  Goat

• 经测试应用

  适用于: Flow Cyt (Intra), ICC/IFmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Synthetic peptide corresponding to TRIM21/SS-A aa 463-475 (N terminal).
  Sequence:

  CPLNIGSQGSTDY

  
  (Peptide available as ab23028)

  Run BLAST with Run BLAST with 

• 阳性对照

• ICC/IF: U2OS cells. Flow Cyt (intra): HeLa cells.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

• 存储溶液

  pH: 7.30
  Preservative: 0.02% Sodium azide
  Constituents: Tris buffered saline, 0.5% BSA

• 浓度

• 100 µg 浓度为 0.5 mg/ml

• 纯度

  Immunogen affinity purified

• 纯化说明

  Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab4369于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 6737 Human

• Omim: 109092 Human

• SwissProt: P19474 Human

• Unigene: 532357 Human

• 别名

• Autoantigen Ro/SSA, 52-KD antibody

• E3 ubiquitin-protein ligase TRIM21 antibody

• RING finger protein 81 antibody

• RNF81 antibody

• Ro 52 antibody

• Ro(SS-A) antibody

• Ro52 antibody

• RO52_HUMAN antibody

• Sicca syndrome antigen A antibody

• Sjoegren syndrome type A antigen antibody

• Sjogren syndrome antigen A1 antibody

• Sjogren syndrome type A antigen antibody

• SS-A antibody

• SSA antibody

• SSA1: Sjogren syndrome antigen A1 (52kDa ribonucleoprotein autoantigen SS-A/Ro) antibody

• TRIM21 antibody

• Tripartite motif protein TRIM21 antibody

• Tripartite motif-containing 21 antibody

• Tripartite motif-containing protein 21 antibody

• 52 kDa ribonucleoprotein autoantigen Ro/SS-A antibody

• 52 kDa Ro protein antibody

• 52kD Ro/SSA autoantigen antibody

• 

  Immunocytochemistry/ Immunofluorescence - Anti-TRIM21/SS-A antibody (ab4369)

  Immunocytochemistry/Immunofluorescence analysis of U2OS cells labelling TRIM21/SS-A with ab4369 at 10 µg/ml. Cells were paraformaldehyde fixed and permeabilized with 0.15% triton. Primary antibody icubation was for 1 hour followed by incubation with an Alexa Fluor® 488-conjugated secondary antibody at 2 µg/ml. DAPI nuclear counterstain was used.

  Negative control: Unimmunized goat IgG (10 µg/ml) followed by an Alexa Fluor® 488-conjugated secondary antibody (2 µg/ml).

• 

  Flow Cytometry (Intracellular) - Anti-TRIM21/SS-A antibody (ab4369)

  Flow cytometry analysis of HeLa cells labelling TRIM21/SS-A with ab4369 (blue line) at 10 µg/ml. Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton. Incubated with the primary antibody for 1 hour followed by incubation with Alexa Fluor® 488-conjugated secondary antibody at 1 µg/ml.

  IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor® 488-conjugated secondary antibody.