Anti-MCM6 antibody

• 产品名称

  Anti-MCM6抗体
  参阅全部 MCM6 一抗

• 描述

  兔多克隆抗体to MCM6

• 宿主

  Rabbit

• 经测试应用

  适用于: WB, IHC-Pmore details

• 种属反应性

  与反应: Human
  预测可用于: Rat, Horse, Guinea pig, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan

• 免疫原

  Synthetic peptide corresponding to Human MCM6.
  Database link: Q14566

• 阳性对照

• WB: RIPA extract from HeLa whole cell lysate. IHC-P: Human breast carcinoma tissue and human skin tissue.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

• 存储溶液

  pH: 7
  Preservative: 0.1% Sodium azide
  Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris

• 纯度

  Immunogen affinity purified

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab4458于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 517812 Cow

• Entrez Gene: 4175 Human

• Entrez Gene: 29685 Rat

• Omim: 601806 Human

• SwissProt: Q2KIZ8 Cow

• SwissProt: Q14566 Human

• SwissProt: Q62724 Rat

• Unigene: 444118 Human

• Unigene: 33226 Rat

别名

• DNA replication licensing factor MCM6 antibody

• MCG40308 antibody

• Mcm6 antibody

• MCM6 minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe) antibody

• MCM6 minichromosome maintenance deficient 6 antibody

• MCM6_HUMAN antibody

• Minichromosome maintenance complex component 6 antibody

• Minichromosome maintenance deficient (mis5 S. pombe) 6 antibody

• Minichromosome maintenance deficient 6 homolog antibody

• Minichromosome maintenance protein 6 antibody

• Mis5 antibody

• MIS5 homolog antibody

• p105MCM antibody

• 

  Western blot - Anti-MCM6 antibody (ab4458)

  Anti-MCM6 antibody (ab4458) at 0.2 µg/ml + RIPA extract from HeLa whole cell lysate at 50 µg
  
  Predicted band size: 91 kDa
  
  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM6 antibody (ab4458)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse squamous cell carcinoma (right) tissues labelling MCM6 with ab4458 at 1/1000 (1µg/ml). Detection: DAB.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM6 antibody (ab4458)

  IHC image of ab4458 staining in normal human skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab4458, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.