Anti-Ninein/NIN抗体- Centrosome Marker
参阅全部 Ninein/NIN 一抗
兔多克隆抗体to Ninein/NIN - Centrosome Marker
Rabbit
适用于: ICC/IFmore details
与反应: Human
预测可用于: Mouse
Fusion protein corresponding to Human Ninein/NIN. The region used shows good homology to Mouse Ninein/NIN aa 1-550.
ICC/IF: HeLa cells
The centrosomal protein ninein has been identified as a microtubule minus end capping and centriole anchoring protein, but its true physiological function remains to be determined. At mitosis, ninein has been observed in association with the mitotic spindle as well as at the centrosomes.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Protein A purified
The centrosomal protein ninein has been identified as a microtubule minus end capping and centriole anchoring protein, but its true physiological function remains to be determined. At mitosis, ninein has been observed in association with the mitotic spindle as well as at the centrosomes.
多克隆
IgG
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“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (2) | 1/500. |
Entrez Gene: 51199 Human
Entrez Gene: 18080 Mouse
Omim: 608684 Human
SwissProt: Q8N4C6 Human
SwissProt: Q61043 Mouse
Unigene: 310429 Human
Unigene: 569387 Human
Unigene: 244047 Mouse
h Glycogen synthase kinase 3 beta-interacting protein antibody
hNinein antibody
KIAA1565 antibody
NIN antibody
NIN protein antibody
NIN_HUMAN antibody
ninein (GSK3B interacting protein) antibody
Ninein antibody
Ninein centrosomal protein antibody
SCKL7 antibody
Centrosome Marker antibody
Glycogen synthase kinase 3 beta interacting protein antibody
GSK3B interacting protein antibody
Immunocytochemistry/ Immunofluorescence - Anti-Ninein/NIN antibody - Centrosome Marker (ab4447)
ab4447 staining Ninein in HeLa cells. The cells were fixed with 4% PFA (10 mins), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab4447 (shown in green) at 0.1µg/ml and ab7291 (shown in red), Mouse monoclonal to alpha Tubulin, at 1µg/ml. The secondary antibodies used were ab150081, Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L), and ab150120, Alexa Fluor® 594 Goat Anti-Mouse IgG (H&L) used at a 1/1000 dilution for 1h at room temperature. Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
Immunocytochemistry/ Immunofluorescence - Anti-Ninein/NIN antibody - Centrosome Marker (ab4447)Gordon Chan, University of Alberta
IF using ab4447 to detect Ninein/NIN in MCF7 cells (human).
The left hand panels are DAPI stained nuclei. The right hand panels are the same nuclei stained for Ninein/NIN.
ab4447 was used at 1/1000.
Ninein/NIN shows nuclear and centrosome staining, which is expected from the literature
