Anti-Cellubrevin抗体
参阅全部 Cellubrevin 一抗
兔多克隆抗体to Cellubrevin
Rabbit
适用于: WB, ICC/IFmore details
与反应: Mouse, Rat, Human
Synthetic peptide corresponding to Mouse Cellubrevin aa 1-18.
Sequence:
MSTGVPSGSSAATGSNRR
(Peptide available as ab5861)
WB: HeLa cell extract (ab7898) ; Mouse Brain ; Rat Brain ICC/IF: NIH/3T3 (Mouse embryonic fibroblast cell line) ; Neuro-2a (Mouse neuroblastoma cell line)
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA
Immunogen affinity purified
The vesicle associated membrane proteins (VAMP) or synaptobrevins are calcium binding proteins specific to eukaryotes. VAMPs, along with synaptosomal associated protein of 25 kDa (SNAP-25) and syntaxin, form the core complex of soluble NSF attachment protein receptor (SNARE) proteins that interact with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and alpha-SNAP. These membrane associated proteins play a key role in the regulation of vesicle membrane fusion with the plasma membrane. The Clostridium tetani neurotoxin is a metalloprotease with specificity for VAMP. In Alzheimer’s disease, all VAMP isoform levels appear to be significantly reduced.
多克隆
IgG
Abpromise™承诺保证使用ab5789于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) | 1/500. Detects a band of approximately 13 kDa (predicted molecular weight: 12 kDa). |
| ICC/IF | 1/50. |
Entrez Gene: 9341 Human
Entrez Gene: 22319 Mouse
Omim: 603657 Human
SwissProt: Q15836 Human
SwissProt: P63024 Mouse
Unigene: 66708 Human
Unigene: 273930 Mouse
Unigene: 219999 Rat
CEB antibody
Cellubrevin antibody
Synaptobrevin 3 antibody
Synaptobrevin-3 antibody
VAMP 3 antibody
VAMP-3 antibody
VAMP3 antibody
VAMP3_HUMAN antibody
Vesicle associated membrane protein 3 antibody
Vesicle-associated membrane protein 3 antibody
Western blot - Anti-Cellubrevin antibody (ab5789)
All lanes : Anti-Cellubrevin antibody (ab5789)
Lane 1 : Mouse Brain
Lane 2 : Rat Brain
Lane 3 : Mouse Liver
Lane 4 : Rat Liver
Predicted band size: 12 kDa
Additional bands at: ~15 kDa. We are unsure as to the identity of these extra bands.
Relative expression of Cellubrevin observed in Mouse Brain and Rat Brain in comparison to Mouse Liver and Rat Liver using ab5789 to demonstrate specificty of expression of target across different tissues.
Western blot - Anti-Cellubrevin antibody (ab5789)
Western blot analysis of recombinant rat VAMP-3 using ab5789.
Western blot - Anti-Cellubrevin antibody (ab5789)
Western blot analysis of VAMP-3 in HeLa cell extract using ab5789.
Immunocytochemistry/ Immunofluorescence - Anti-Cellubrevin antibody (ab5789)
Immunofluorescent analysis of formalin-fixed, 0.1% Triton™ X-100 permeabilized, NIH/3T3 (Mouse embryonic fibroblast cell line) cells, blocked with 3% BSA-PBS for 30 minutes at room temperature. Labelling cellubrevin with ab5789 at 1/50 dilution and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody at room temperature in the dark (right image). Compared with negative control without primary antibody (left image). Nuclei were stained with DAPI (Blue). Actin was stained with Alexa Fluor® 555 (Red). The images were captured at 60X magnification.
Immunocytochemistry/ Immunofluorescence - Anti-Cellubrevin antibody (ab5789)
Immunofluorescent analysis of formalin-fixed, 0.1% Triton™ X-100 permeabilized, Neuro-2a (Mouse neuroblastoma cell line) cells, blocked with 3% BSA-PBS for 30 minutes at room temperature. Labelling cellubrevin with ab5789 at 1/50 dilution and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody at room temperature in the dark (right image). Compared with negative control without primary antibody (left image). Nuclei were stained with DAPI (Blue).The images were captured at 60X magnification.
