Human Histone H2B peptide

• 产品名称

  人Histone H2B多肽
  参阅全部 Histone H2B 蛋白酶

• 纯度

  > 90 % HPLC.
  
  

• 
  

• 无动物成分

  No

• 性质

  Synthetic

• 
  

• 种属

  Human

Our Abpromise guarantee covers the use of ab16101 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

• 应用

  Blocking - Blocking peptide for Anti-Histone H2B antibody - ChIP Grade (ab1790), Anti-Histone H2B antibody [mAbcam 52484] - ChIP Grade (ab52484), Anti-Histone H2B antibody [mAbcam 64165] - ChIP Grade (ab64165)

• 形式

  Liquid

• 补充说明

  - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
  - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
  - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
  - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
  - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

• 浓度

• 1 mg 浓度为 1 mg/ml

• 稳定性和存储

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  Information available upon request.

别名

• GL105

• H2B GL105

• H2B histone family member O

• H2B histone family member S

• H2B.1

• H2B.1 B

• H2B.b

• H2B.c

• H2B.d

• H2B.e

• H2B.f

• H2B.j

• H2B.q

• H2B/b

• H2B/c

• H2B/d

• H2B/e

• H2B/f

• H2B/j

• H2B/o

• H2B/q

• H2BFB

• H2BFC

• H2BFD

• H2BFE

• H2BFF

• H2BFJ

• H2BFO

• H2BFQ

• H2BFS

• H2BGL105

• H2BQ

• HIRIP2

• HIST1H2BB

• HIST1H2BD

• HIST1H2BH

• HIST1H2BL

• HIST1H2BM

• HIST1H2BN

• HIST2H2BE

• histone cluster 2

• Histone H2B

• histone H2B GL105

• Histone H2B type 1 B

• Histone H2B type 1 D

• Histone H2B type 1 H

• Histone H2B type 1 L

• Histone H2B type 1 M

• Histone H2B type 1 N

• Histone H2B type 2 E

• Histone H2B.q

• histone protein

• 

  Western blot - Human Histone H2B peptide (ab16101)

  All lanes : Anti-Histone H2B antibody - ChIP Grade (ab1790) at 0.1 µg/ml
  
  Lane 1 : Calf thymus histone prep
  Lane 2 : Calf thymus histone prep with Human Histone H2B peptide (ab16101) at 1 µg/ml
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  All lanes : Alexa fluor Goat polyclonal anti-Rabbit IgG at 1/10000 dilution
  
  Performed under reducing conditions.