Anti-V5 tag抗体
参阅全部 V5 tag 一抗
兔多克隆抗体to V5 tag
Rabbit
适用于: ChIP, WBmore details
与反应: Species independent
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
WB: Recombinant V5 tagged Groucho homolog. ChIP: A stably transfected 293T human cell line harbouring the GAL4 upstream activation sequence was transiently transfected with a V5 or T7- tagged GAL4 DNA Binding Domain construct.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
浓度
批次浓度范围 100 µg 浓度为 0.9 - 1 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab15828于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ChIP | Use at an assay dependent concentration. ChIP was performed with 25 ug chromatin, 5ug of antibody and 20 ul of Protein A/G beads. | |
| WB | (1) | Use a concentration of 0.5 µg/ml. |
GKPIPNPLLGLDST epitope tag antibody
GKPIPNPLLGLDST tag antibody
Protein Rev antibody
Regulator of expression of viral proteins antibody
rev antibody
V5 epitope tag antibody
ChIP - Anti-V5 tag antibody (ab15828)
A stably transfected 293T human cell line harbouring the GAL4 upstream activation sequence was transiently transfected with a V5 or T7- tagged GAL4 DNA Binding Domain construct. 48 hours post transfection Chromatin was prepared according to the Abcam X-ChIP protocol. The ChIP was performed with 25 ug chromatin, 5ug of antibody and 20 ul of Protein A/G beads. A non-specific antibody was used as the negative control. The immunoprecipitated DNA was quantified by real time PCR (SYBR Green approach).
Western blot - Anti-V5 tag antibody (ab15828)
All lanes : Anti-V5 tag antibody (ab15828) at 0.5 µg/ml
Lane 1 : Recombinant V5 tagged Groucho homolog
Lane 2 : Recombinant V5 tagged Groucho with V5 tag peptide (ab15829) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG at 1/10000 dilution
Performed under reducing conditions.
