首页 > 产品目录 > 免疫学 > 抗体 > Anti-FOXP1 antibody

Anti-FOXP1 antibody

产品编号 : ab16645

英文名称 :

中文名称 : 抗体

产品等级 :

品牌 : Abcam

包装规格	交货周期	质量标准	目录价	会员专享价	数量
100ug			￥5430	登录后可见	- +

产品详情

产品名称
Anti-FOXP1抗体
参阅全部 FOXP1 一抗
描述
兔多克隆抗体to FOXP1
宿主
Rabbit
特异性
Replenishment batches of our polyclonal antibody, ab16645 are tested in IHC-P. Previous batches were additionally validated in IHC-FoFr and WB. These applications are still expected to work and are covered by our Abpromise guarantee. You may also be interested in our alternative recombinant antibody, ab134055.
经测试应用
适用于: IHC-FoFr, IHC-P, WBmore details
种属反应性
与反应: Mouse
预测可用于: Rat, Chicken, Dog, Human, Xenopus laevis
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
阳性对照

常规说明

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
形式
Liquid
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA

Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
浓度

Abpromise™承诺保证使用ab16645于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
IHC-FoFr		Use at an assay dependent concentration.
IHC-P	(4)	Use a concentration of 0.1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB	(7)	1/5000. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa). ab16645 recognises the different isoforms of FOXP1 in Jurkat cell lysate (see review for further details).

应用

Ab评论

说明

IHC-FoFr

Use at an assay dependent concentration.

IHC-P

Use a concentration of 0.1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

1/5000. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).

ab16645 recognises the different isoforms of FOXP1 in Jurkat cell lysate (see review for further details).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP1 antibody (ab16645)
Paraffin-embedded mouse brain tissue stained for FOXP1 using ab16645 in immunohistochemical analysis.
Western blot - Anti-FOXP1 antibody (ab16645)
All lanes : Anti-FOXP1 antibody (ab16645) at 1/5000 dilution

Lane 1 : Lysate from e12.5 whole mouse embryo FoxP1 +/-
Lane 2 : Lysate from e12.5 whole mouse embryo FoxP1 -/-

Lysates/proteins at 30 µg per lane.

Developed using the ECL technique.

Predicted band size: 75 kDa
Observed band size: 65,82 kDawhy is the actual band size different from the predicted?
Additional bands at: 45 kDa (possible cross reactivity), 50 kDa (possible cross reactivity), 85 kDa (possible cross reactivity)
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-FOXP1 antibody (ab16645)This image is courtesy of Shunyou Wang, Ph.D.
ab16645 was tested in IHC of formalin fixed sections from mouse (over two weeks of age) brain. Antigen retrieval was in citrate buffer and blocking was in 1% BSA. The primary antibody was incubated at a dilution of 1/200 overnight at 4 degrees celsius. The secondary antibody was a biotin conjugated anti-rabbit IgG.