Anti-FOXP2抗体
参阅全部 FOXP2 一抗
兔多克隆抗体to FOXP2
Rabbit
From September 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
适用于: WB, ICC/IF, IHC-Pmore details
与反应: Mouse
预测可用于: Rat
不与反应: Human
Synthetic peptide corresponding to Human FOXP2 aa 700 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available as ab16278)
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
浓度
批次浓度范围 100 µg 浓度为 0.9 - 1 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab16046于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (3) | 1/1000. |
| ICC/IF | (2) | Use at an assay dependent concentration. |
| IHC-P | (4) | Use at an assay dependent concentration. |
Entrez Gene: 114142 Mouse
SwissProt: P58463 Mouse
Unigene: 332919 Mouse
Unigene: 225249 Rat
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP2 antibody (ab16046)
IHC image of FOXP2 staining in mouse e17 foetal brain formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16046, 0.1µg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Immunocytochemistry/ Immunofluorescence - Anti-FOXP2 antibody (ab16046)This image is courtesy of an abreview submitted by Jeremy Dasen.
Mouse spinal cord was fixed in paraformaldehyde, blocked in 1% BSA for 30 minutes then incubated with ab16046 at 1/8000 dilution for 18 hours. This image was submitted as part of a review by Jeremy Dasen.
