Human gamma H2A.X (phospho S139) peptide

• 产品名称

  人gamma H2A.X (phospho S139)多肽

• 纯度

  > 90 % HPLC.
  
  

• Accession

  P16104

• 无动物成分

  No

• 性质

  Synthetic

• 
  

• 种属

  Human

• 修饰

  phospho S139

Our Abpromise guarantee covers the use of ab15645 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

• 应用

  Blocking - Blocking peptide for Anti-gamma H2A.X (phospho S139) antibody (ab2893)

• 形式

  Lyophilized

• 补充说明

  - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
  - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
  - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
  - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
  - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

• 稳定性和存储

  Shipped at 4°C. Store at -20°C.

  Information available upon request.

别名

• H2A histone family member X

• H2A.FX

• H2A.X

• H2a/x

• H2AFX

• H2AX

• H2AX_HUMAN

• Histone H2A.X

• 

  Western blot - Human gamma H2A.X (phospho S139) peptide (ab15645)

  All lanes : Anti-gamma H2A.X (phospho S139) antibody (ab2893) at 1/500 dilution
  
  Lane 1 : Control HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate Histone preparation
  Lane 2 : Colcemid treated HeLa whole cell lysate Histone preparation
  Lane 3 : Control HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate Histone preparation with Human gamma H2A.X (phospho S139) peptide (ab15645) at 1 µg
  Lane 4 : Colcemid treated HeLa whole cell lysate Histone preparation with Human gamma H2A.X (phospho S139) peptide (ab15645) at 1 µg
  Lane 5 : Control HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate Histone preparation with Human Histone H2A.X (unmodified ) peptide (ab15646) at 1 µg
  Lane 6 : Colcemid treated HeLa whole cell lysate Histone preparation with Human Histone H2A.X (unmodified ) peptide (ab15646) at 1 µg
  
  Secondary
  All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution