Anti-TGF beta 3抗体
参阅全部 TGF beta 3 一抗
兔多克隆抗体to TGF beta 3
Rabbit
适用于: IP, WB, ICC/IF, IHC-Pmore details
与反应: Mouse, Rat, Rabbit, Human
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab15537于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP | (1) | Use at an assay dependent concentration. |
| WB | (2) | Use at an assay dependent concentration. Predicted molecular weight: 47 kDa. The immunogen for ab15537 is located close to C-terminus and therefore should be able to detect the proform at 50 kDa and mature form at 12.5 kDa under reducing condition. |
| ICC/IF | Use a concentration of 5 µg/ml. | |
| IHC-P | (2) | 1/100. Incubate for 10 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at RT for 20 minutes. Ab15537 demonstrates cytoplasmic staining. |
hide
Unigene: 3992 Mouse
Unigene: 7018 Rat
Entrez Gene: 7043 Human
Entrez Gene: 21809 Mouse
Omim: 190230 Human
SwissProt: P10600 Human
SwissProt: P17125 Mouse
Unigene: 592317 Human
LDS5 antibody
MGC105479 antibody
MGC118722 antibody
prepro-transforming growth factor beta-3 antibody
RNHF antibody
TGF beta 3 antibody
TGF beta3 antibody
TGF-beta-3 antibody
TGFB 3 antibody
Tgfb3 antibody
TGFB3_HUMAN antibody
transforming growth factor beta 3 antibody
Transforming growth factor beta-3 antibody
ARVD antibody
ARVD1 antibody
FLJ16571 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGF beta 3 antibody (ab15537)
Human placenta stained with ab15537 by Immunohistochemistry (FFPE-sections).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGF beta 3 antibody (ab15537)This image is courtesy of an anonymous Abreview
ab15537 at 1/200 staining human anal cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed before incubation with the antibody for 30 minutes. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
Immunocytochemistry/ Immunofluorescence - Anti-TGF beta 3 antibody (ab15537)
ICC/IF image of ab15537 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab15537 5µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
