Anti-Presenilin 1/PS-1 antibody [APS 11]

• 产品名称

  Anti-Presenilin 1/PS-1抗体[APS 11]
  参阅全部 Presenilin 1/PS-1 一抗

• 描述

  小鼠单克隆抗体[APS 11] to Presenilin 1/PS-1

• 宿主

  Mouse

• 特异性

  No cross-reactivity is seen with presenilin 2. In formalin-fixed, paraffin embedded sections of human brain, this antibody showed strong staining of both the plaque core and dystrophic neurites. By Western blot, this antibody detects an ~28 kDa protein representing PS1 N-terminus cleavage product in ST15 cell lysate transfected with human PS1.

• 经测试应用

  适用于: ICC/IF, IHC-P, Flow Cytmore details

• 种属反应性

  与反应: Mouse, Human
  预测可用于: Cow, Dog, Non human primates, Cynomolgus monkey

• 免疫原

  Synthetic peptide corresponding to Human Presenilin 1/PS-1 aa 1-100.
  Database link: P49768

  Run BLAST with Run BLAST with 

• 阳性对照

• IHC: Human brain tissue slides. WB: ST15 cell lysate transfected with human PS1. ICC/IF: mouse fibroblast

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.05% Sodium azide
  Constituents: 99% PBS, 0.1% BSA

• 浓度

• 200 µg 浓度为 1 mg/ml

• 纯度

  Protein G purified

• 克隆

  单克隆

• 克隆编号

  APS 11

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab15456于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 5663 Human

• Entrez Gene: 19164 Mouse

• Omim: 104311 Human

• SwissProt: P49768 Human

• SwissProt: P49769 Mouse

• Unigene: 3260 Human

• Unigene: 998 Mouse

• 别名

• Homo Sapiens Clone CC44 Senilin 1 antibody

• Presenilin-1 CTF12 antibody

• Protein S182 antibody

• PS 1 antibody

• PS-1 antibody

• PS1-CTF12 antibody

• PSEN1 antibody

• PSN1_HUMAN antibody

• PSNL1 antibody

• S182 antibody

• AD3 antibody

• Ad3h antibody

• FAD antibody

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Presenilin 1/PS-1 antibody [APS 11] (ab15456)

  Immunofluorescent staining of Presenilin-1/PS1 in mouse fibroblast fixed with methanol, incubated with ab15456 at 0.3 ug/ul of an antibody concentration of 11.2 mg/mL (dilution 1/37). The experiment was performed with transient transfected mouse fibroblast cells.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Presenilin 1/PS-1 antibody [APS 11] (ab15456)

  Immunohistochemistry was performed on normal biopsies of deparaffinized Human liver tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Presenilin 1 ab15456 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
  
  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Presenilin 1/PS-1 antibody [APS 11] (ab15456)

  Immunohistochemistry was performed on normal biopsies of deparaffinized Human brain tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a mouse monoclonal antibody recognizing Presenilin 1 ab15456 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
  
  

• 

  Flow Cytometry - Anti-Presenilin 1/PS-1 antibody [APS 11] (ab15456)

  Overlay histogram showing HepG2 cells stained with ab15456 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab15456, 1ug/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG1 (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min) permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.