Anti-SEC61B antibody

• 产品名称

  Anti-SEC61B抗体
  参阅全部 SEC61B 一抗

• 描述

  兔多克隆抗体to SEC61B

• 宿主

  Rabbit

• 特异性

  Ab15576 detects human, dog, pig, and rat sec61 beta from microsomes and shows no cross-reactivity with other sec61 isoforms.

• 经测试应用

  适用于: ICC/IFmore details

• 种属反应性

  与反应: Mouse, Rat, Dog, Human, Pig
  

• 免疫原

  Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

• 阳性对照

• ICC/IF: MDCK cells.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.05% Sodium azide

• 纯度

  Whole antiserum

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab15576于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

  hide

• Unigene: 191887 Human

• Unigene: 20188 Mouse

• Entrez Gene: 404018 Dog

• Entrez Gene: 10952 Human

• Entrez Gene: 66212 Mouse

• Entrez Gene: 298068 Rat

• Omim: 609214 Human

• SwissProt: P60467 Dog

• SwissProt: P60468 Human

• SwissProt: Q9CQS8 Mouse

• 别名

• SC61B_HUMAN antibody

• SEC 61B antibody

• SEC61 B antibody

• Sec61 beta subunit antibody

• Sec61 complex beta subunit antibody

• SEC61B antibody

• Protein translocation complex beta antibody

• Protein transport protein SEC61 beta subunit antibody

• Protein transport protein Sec61 subunit beta antibody

• 

  Immunocytochemistry/ Immunofluorescence - Anti-SEC61B antibody (ab15576)

  Immunofluorescence analysis of SEC61B was performed using 70% confluent log phase MDCK cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with SEC61B Rabbit Polyclonal Antibody (ab15576) at 1/250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1/2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Rhodamine Phalloidin. Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.