Anti-MRP4 antibody [M4I-10]

• 产品名称

  Anti-MRP4抗体[M4I-10]
  参阅全部 MRP4 一抗

• 描述

  大鼠单克隆抗体[M4I-10] to MRP4

• 宿主

  Rat

• 经测试应用

  适用于: IHC-P, WBmore details

• 种属反应性

  与反应: Mouse, Human
  

• 免疫原

  Recombinant fragment (MBP-tag) corresponding to Human MRP4 aa 350-450. Fusion protein containing the E. coli maltose binding protein and a fragment of the human MRP4 protein corresponding to amino acids 372-431.

• 阳性对照

• Kidney tissue This antibody gave a positive result in IHC in the following FFPE tissue: Human lung adenocarcinoma.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.30
  Preservative: 0.02% Sodium azide
  Constituents: 0.1% BSA, PBS

• 纯度

  Tissue culture supernatant

• 克隆

  单克隆

• 克隆编号

  M4I-10

• 同种型

  IgG2a

The Abpromise guarantee

Abpromise™承诺保证使用ab15602于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 10257 Human

• Entrez Gene: 239273 Mouse

• Omim: 605250 Human

• SwissProt: O15439 Human

• Unigene: 508423 Human

• 别名

• ATP binding cassette sub family C member 4 antibody

• ATP-binding cassette sub-family C member 4 antibody

• bA464I2.1 (ATP binding cassette, sub-family C (CFTR/MRP) member 4) antibody

• bA464I2.1 antibody

• Canalicular multispecific organic anion transporter antibody

• Canalicular multispecific organic anion transporter ABC superfamily antibody

• EST170205 antibody

• MOAT B antibody

• MOAT-B antibody

• MOATB antibody

• MRP 4 antibody

• MRP/cMOAT related ABC transporter antibody

• MRP/cMOAT-related ABC transporter antibody

• MRP4_HUMAN antibody

• Multi specific organic anion transporter B antibody

• Multi-specific organic anion transporter B antibody

• Multidrug resistance associated protein 4 antibody

• Multidrug resistance-associated protein 4 antibody

• OTTHUMP00000018560 antibody

• ABCC 4 antibody

• ABCC4 antibody

• ATP binding cassette sub family C (CFTR/MRP) member 4 antibody

• 

  Western blot - Anti-MRP4 antibody [M4I-10] (ab15602)

  All lanes : Anti-MRP4 antibody [M4I-10] (ab15602) at 20 µg/ml
  
  Lane 1 : Wild-type HAP1 whole cell lysate
  Lane 2 : ABCC4 (MRP4) knockout HAP1 whole cell lysate
  
  Lysates/proteins at 20 µg per lane.
  
  Predicted band size: 159 kDa
  
  
  
  

  Lanes 1 - 2: Merged signal (red and green). Green - ab15602 observed at 200-250 kDa. Red - loading control, ab176560, observed at 50 kDa.
  
  ab15602 was shown to specifically react with MRP4 in wild-type HAP1 cells as signal was lost in ABCC4 (MRP4) knockout cells. Wild-type and ABCC4 (MRP4) knockout samples were subjected to SDS-PAGE. Ab15602 and ab176560 (Rabbit anti-alpha Tubulin loading control) were incubated overnight at 4°C at 20 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rat IgG H&L (IRDye® 800CW) (ab253031) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP4 antibody [M4I-10] (ab15602)

  HC image of MRP4 staining in Human lung adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab15602, 10µg/ml, for 15 mins at room temperature. A Goat anti-Rat biotinylated secondary antibody (ab253031) was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.