Anti-Drosha抗体
参阅全部 Drosha 一抗
兔多克隆抗体to Drosha
Rabbit
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
适用于: WB, ICCmore details
与反应: Mouse, Human
Synthetic peptide corresponding to Human Drosha aa 1-100 (internal sequence).
(Peptide available as ab12307)
Three isoforms of 159, 156 and 143 kDa.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
浓度
批次浓度范围 100 µg 浓度为 0.9 - 1 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab12286于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (4) | Use a concentration of 1 µg/ml. Predicted molecular weight: 159 kDa. |
| ICC | (1) | Use a concentration of 1 µg/ml. |
Entrez Gene: 29102 Human
Entrez Gene: 14000 Mouse
Omim: 608828 Human
SwissProt: Q9NRR4 Human
SwissProt: Q5HZJ0 Mouse
Unigene: 97997 Human
Unigene: 293142 Mouse
Etohi2 antibody
HSA242976 antibody
Nuclear RNase III Drosha antibody
p241 antibody
Protein Drosha antibody
Putative protein p241 which interacts with transcription factor Sp1 antibody
Putative ribonuclease III antibody
RANSE3L antibody
Ribonuclease 3 antibody
Ribonuclease III antibody
Ribonuclease III nuclear antibody
Ribonuclease type III nuclear antibody
RibonucleaseIII antibody
RN 3 antibody
RN3 antibody
RNase 3 antibody
RNase III antibody
RNase3 antibody
RNASE3L antibody
RNaseIII antibody
RNASEN antibody
RNC_HUMAN antibody
DROSHA antibody
Drosha double stranded RNA specific endoribonuclease antibody
Drosha ribonuclease type III antibody
Immunocytochemistry - Anti-Drosha antibody - ChIP Grade (ab12286)This image is courtesy of Ahmad M. Khalil and Daniel J. Driscoll, University of Florida College of Medicine Genetics Institute.
Human female amniocytes immunostained with ab12286 Drosha (FITC) (1/25 dilution). The DNA is labelled red with propidium iodide. This image was submitted as part of a review by Ahmad Khalil.
Western blot - Anti-Drosha antibody - ChIP Grade (ab12286)
All lanes : Anti-Drosha antibody (ab12286) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 159 kDa
Observed band size: 170 kDawhy is the actual band size different from the predicted?
Immunocytochemistry - Anti-Drosha antibody (ab12286)
ab12286 at a 1/25 dilution staining human HeLa cells by immunocytochemistry. The antibody was incubated with the cells for 1 hour and then detected using a Cy3 conjugated donkey anti-rabbit polyclonal antibody.
This image is courtesy of an Abreview submitted on 7 February 2006.
Western blot - Anti-Drosha antibody - ChIP Grade (ab12286)
All lanes : Anti-Drosha antibody (ab12286) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Nuclear Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 159 kDa
Observed band size: 170 kDawhy is the actual band size different from the predicted?
Additional bands at: 115 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
Immunocytochemistry - Anti-Drosha antibody (ab12286)
ICC/IF image of ab12286 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12286, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunocytochemistry - Anti-Drosha antibody (ab12286)
ICC/IF image of ab12286 stained Hela cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12886, 5µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
抱歉，暂无浏览记录
