Anti-Slow Skeletal Myosin Heavy chain抗体[NOQ7.5.4D]
参阅全部 Slow Skeletal Myosin Heavy chain 一抗
小鼠单克隆抗体[NOQ7.5.4D] to Slow Skeletal Myosin Heavy chain
Mouse
适用于: IHC-P, WBmore details
与反应: Rat, Rabbit
预测可用于: Sheep, Goat, Chicken, Guinea pig, Hamster, Cow, Cat, Dog, Human, Pig
Full length native protein (purified) corresponding to Human Slow Skeletal Myosin Heavy chain. Human skeletal muscle myosin purified from myofibrils.
IHC-P: Rabbit tongue tissue. WB: Rat skeletal muscle tissue extract.
This product was changed from ascites to tissue culture supernatant on 25th October 2016. The following lot(s) is/are from ascites and is still in stock as of 25th October 2016- GR201056, GR231200, GR285981. Lot numbers other than GR201056, GR231200, GR285981will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS
浓度
批次浓度范围 50 µl 浓度为 1 - 10 mg/ml
Proprietary Purification
Purified from Tissue culture supernatant.
单克隆
NOQ7.5.4D
IgG1
Abpromise™承诺保证使用ab11083于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P | (5) | Use a concentration of 2.5 - 5 µg/ml. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. |
WB | (1) | Use a concentration of 1.25 - 2.5 µg/ml. Detects a band of approximately 200 kDa (predicted molecular weight: 200 kDa). |
Entrez Gene: 4625 Human
Omim: 160760 Human
SwissProt: P12883 Human
Unigene: 719946 Human
Unigene: 225886 Rat
Unigene: 54399 Rat
CMH1 antibody
MPD1 antibody
MYH7 antibody
MYH7_HUMAN antibody
Myhc slow antibody
MyHC-beta antibody
MyHC-slow antibody
MYHCB antibody
Myopathy, distal 1 antibody
Myosin heavy chain (AA 1-96) antibody
Myosin heavy chain 7 antibody
Myosin heavy chain antibody
Myosin heavy chain slow isoform antibody
Myosin heavy chain, cardiac muscle beta isoform antibody
Myosin, heavy chain 7, cardiac muscle, beta antibody
Myosin, heavy polypeptide 7, cardiac muscle, beta antibody
Myosin-7 antibody
Rhabdomyosarcoma antigen MU RMS 40.7A antibody
SPMD antibody
SPMM antibody
Beta myosin heavy chain antibody
cardiac muscle beta isoform antibody
CMD1S antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [NOQ7.5.4D] (ab11083)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded rabbit tomgue tissue staining Skeletal Myosin Heavy chain using ab11083 at 2.5 μg/mL.
Western blot - Anti-Slow Skeletal Myosin Heavy chain antibody [NOQ7.5.4D] (ab11083)
Lane 1 : Anti-Slow Skeletal Myosin Heavy chain antibody [NOQ7.5.4D] (ab11083) at 1.25 µg/ml
Lane 2 : Anti-Slow Skeletal Myosin Heavy chain antibody [NOQ7.5.4D] (ab11083) at 2.5 µg/ml
Lane 3 : Negative control antibody
All lanes : Rat skeletal muscle
Secondary
All lanes : Anti-Mouse IgG (Fac-specific)-peroxidase conjugate at 1/5000 dilution
Predicted band size: 200 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [NOQ7.5.4D] (ab11083)
ab11083 at 1/500 dilution, staining Slow Skeletal Myosin Heavy chain in mouse tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [NOQ7.5.4D] (ab11083)
ab11083 at 1/4000 dilution, staining Slow Skeletal Myosin Heavy chain in rabbit tongue tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin embedded