Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker

• 产品名称

  Anti-gamma Tubulin抗体[GTU-88] - Centrosome Marker
  参阅全部 gamma Tubulin 一抗

• 描述

  小鼠单克隆抗体[GTU-88] to gamma Tubulin - Centrosome Marker

• 宿主

  Mouse

• 经测试应用

  适用于: ICC/IF, WBmore details

• 种属反应性

  与反应: Mouse, Chicken, Cow, Dog, Human, African green monkey, Chinese hamster
  

• 免疫原

  Synthetic peptide conjugated to KLH, corresponding to N-terminal amino acids 38-53 of gamma Tubulin.

• 常规说明

  This product was changed from ascites to tissue culture supernatant on 18th October 2016. The following lots are from ascites and are still in stock as of 18th October 2016 (GR286332 and GR225007). Lot numbers higher than GR286332 will be from tissue culture supernatant. Please note that dilutions may need to be adjusted accordingly.

  Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

• 存储溶液

  pH: 7.40
  Preservative: 0.097% Sodium azide
  Constituent: PBS

• 纯度

  Proprietary Purification

• 纯化说明

  Purified from Tissue Culture Supernatant.

• 克隆

  单克隆

• 克隆编号

  GTU-88

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab11316于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 540216 Cow

• Entrez Gene: 541101 Cow

• Entrez Gene: 403695 Dog

• Entrez Gene: 490962 Dog

• Entrez Gene: 27175 Human

• Entrez Gene: 7283 Human

• Entrez Gene: 103733 Mouse

• Entrez Gene: 103768 Mouse

• Omim: 191135 Human

• Omim: 605785 Human

• SwissProt: Q0VCD2 Cow

• SwissProt: Q32KM1 Cow

• SwissProt: Q9GKK5 Dog

• SwissProt: P23258 Human

• SwissProt: Q9NRH3 Human

• SwissProt: P83887 Mouse

• SwissProt: Q8VCK3 Mouse

• Unigene: 708059 Human

• Unigene: 142348 Mouse

别名

• Gamma 1 tubulin antibody

• Gamma 2 tubulin antibody

• Gamma Tubulin 1 antibody

• Gamma Tubulin 2 antibody

• Gamma tubulin complex component 1 antibody

• Gamma-2-tubulin antibody

• GCP 1 antibody

• GCP-1 antibody

• GCP1 antibody

• MGC131994 antibody

• TBG2_HUMAN antibody

• TUBG antibody

• TUBG1 antibody

• TUBG2 antibody

• TUBGCP1 antibody

• Tubulin gamma 1 chain antibody

• Tubulin gamma 2 chain antibody

• Tubulin gamma complex-associated protein 1 antibody

• Tubulin gamma-2 chain antibody

• tubulin, gamma 1 antibody

• tubulin, gamma 2 antibody

• tubulin, gamma polypeptide antibody

• Xgam antibody

• 

  Western blot - Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker (ab11316)

  All lanes : Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker (ab11316) at 1/10000 dilution
  
  Lane 1 : HeLa cell line lysates
  Lane 2 : U87 cell line lysates
  Lane 3 : COS7 cell line lysates
  Lane 4 : P19 cell line lysates
  Lane 5 : Hepa1-6 cell line lysates
  Lane 6 : CHO cell line lysates
  Lane 7 : MDBK cell line lysates
  Lane 8 : MDCK cell line lysates
  
  Secondary
  All lanes : Goat Anti-Mouse IgG-Peroxidase
  
  Predicted band size: 48 kDa
  
  

• 

  Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker (ab11316)

  ab11316 at a 1/200 dilution staining HeLa cells by Immunocytochemistry. The antibody was incubated with the cells for 30 minutes and then detected using a Cy3 conjugated Goat anti-Mouse (H+L) (green stain). The antibody recognizes gamma-tubulin located at the centrosomes in metaphase and also faintly recognizes the centrosome association during interphase.

  This image is courtesy of an Abreview submitted by Kirk McManus on 2 December 2005.

  See Abreview

• 

  Western blot - Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker (ab11316)

  Lane 1 : Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker (ab11316) at 1/20000 dilution
  Lane 2 : Negative control (secondary antibody only)
  
  All lanes : Whole cell extract of chicken fibroblasts
  
  Secondary
  All lanes : Alkaline Phosphatase conjugated goat anti-mouse IgG
  
  Predicted band size: 48 kDa
  Observed band size: 48 kDa
  
  

• 

  Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [GTU-88] - Centrosome Marker (ab11316)

  Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling gamma Tubulin using ab11316 at a dilution of 1/5000. Cells were permeabilized with methanol and folowed by fixation with acetone. A Cy3-conjugated goat anti-mouse IgG was used as the secondary antibody.