Anti-RPA32/RPA2抗体
参阅全部 RPA32/RPA2 一抗
兔多克隆抗体to RPA32/RPA2
Rabbit
适用于: WB, IP, IHC-Pmore details
与反应: Mouse, Human
预测可用于: Rabbit, Horse, Pig, Chimpanzee, Rhesus monkey, Gorilla, Bat, Elephant
Synthetic peptide corresponding to Human RPA32/RPA2 aa 200 to the C-terminus (C terminal).
Database link: P15927
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
浓度
50 µg 浓度为 1 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab10359于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | 1/500 - 1/2500. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa). | |
IP | Use at an assay dependent concentration. | |
IHC-P | Use at an assay dependent concentration. |
Entrez Gene: 456680 Chimpanzee
Entrez Gene: 101144875 Gorilla
Entrez Gene: 6118 Human
Entrez Gene: 19891 Mouse
Entrez Gene: 100347550 Rabbit
Entrez Gene: 716612 Rhesus monkey
Omim: 179836 Human
SwissProt: P15927 Human
SwissProt: Q62193 Mouse
Unigene: 703070 Human
Unigene: 79411 Human
Unigene: 2870 Mouse
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Western blot - Anti-RPA32/RPA2 antibody (ab10359)
All lanes : Anti-RPA32/RPA2 antibody (ab10359) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : HEK293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : Mouse TCMK-1 whole cell lysate
Lane 5 : Mouse NIH 3T3 whole cell lysate
Lysates/proteins at 5 µg per lane.
Predicted band size: 32 kDa
Exposure time: 10 seconds
Immunoprecipitation - Anti-RPA32/RPA2 antibody (ab10359)
HeLa whole cell lysate cells treated with 100 µM etoposide for 4 hours (+) or mock treated (-).
Affinity purified rabbit anti-RPA32 antibody (3 µg) and rabbit anti-Phospho RPA32 antibody (1/100 dilution) were used.
Detection: Chemiluminescence
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody (ab10359)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling RPA32/RPA2 with ab10359 at 1/5000 (0.2µg/ml). Detection: DAB.