Anti-Actin抗体[AC-40]
参阅全部 Actin 一抗
小鼠单克隆抗体[AC-40] to Actin
Mouse
适用于: ELISA, IHC-P, IHC-Fr, ICC/IF, WB, IHC-FoFr, Dot blotmore details
与反应: Mouse, Rat, Sheep, Rabbit, Goat, Chicken, Guinea pig, Hamster, Cow, Dog, Human, Pig, Xenopus laevis, Carp, Snail
Synthetic peptide within Human Actin aa 365-375 (C terminal). The exact sequence is proprietary.
Sequence:
SGPSIVHRKCF
Monoclonal anti-Actin recognizes an epitope located on the C-terminal end of actin. This epitope is conserved in all actin isoforms.
Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
This product was changed from ascites to tissue culture supernatant on 21 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS
Tissue culture supernatant
Purified from TCS.
单克隆
AC-40
IgG2a
Abpromise™承诺保证使用ab11003于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ELISA | (1) | Use at an assay dependent concentration. |
IHC-P | Use at an assay dependent concentration. | |
IHC-Fr | Use at an assay dependent concentration. | |
ICC/IF | (1) | Use at an assay dependent concentration. 1/200 determined by indirect immunofluorescent staining of cultured Human or chicken fibroblasts. |
WB | (2) | Use at an assay dependent concentration. Predicted molecular weight: 42 kDa. 1/500 using cultured Human or chicken fibroblast extract. Predicted molecular weight: 42 kDa. |
IHC-FoFr | Use at an assay dependent concentration. | |
Dot blot | Use at an assay dependent concentration. |
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SwissProt: P68133 Human
SwissProt: P68134 Mouse
SwissProt: P68135 Rabbit
Unigene: 1288 Human
Unigene: 214950 Mouse
Unigene: 82732 Rat
Entrez Gene: 421534 Chicken
Entrez Gene: 58 Human
Entrez Gene: 11459 Mouse
Omim: 102610 Human
SwissProt: P68139 Chicken
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Immunohistochemistry (Frozen sections) - Anti-Actin antibody [AC-40] (ab11003)
Immunohistochemical analysis of frozen Human tongue tissue, using ab11003.
This image was generated using the ascites version of the product.