Anti-LYVE1抗体
参阅全部 LYVE1 一抗
兔多克隆抗体to LYVE1
Rabbit
适用于: IHC-Fr, Flow Cyt, IHC-P, WBmore details
与反应: Human
预测可用于: Rat
Recombinant fragment corresponding to Human LYVE1 aa 1-250. Recombinant human soluble LYVE1 fragment produced in insect cells (ab54341).
Database link: Q9Y5Y7
human colon carcinoma
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Constituent: PBS
浓度
100 µg 浓度为 1 mg/ml
Protein A purified
Protein-A Chromatography (+his tag depleted).
The lymphatic vasculature forms a second circulatory system that drains extracellular fluid from the tissues and provides an exclusive environment in which immune cells can encounter and respond to foreign antigen. Recently a number of interesting molecules have been identified that may be exploited as markers for lymphatic endothelium, including the hyaluronan receptor LYVE1, PALE, VEGFR3, podoplanin.
多克隆
IgG
Abpromise™承诺保证使用ab10278于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-Fr | (1) | Use a concentration of 6 - 30 µg/ml. Fix sections for 10 min at -20°C in MeOH. |
| Flow Cyt | (1) | Use at an assay dependent concentration. |
| IHC-P | (2) | Use a concentration of 2 µg/ml. |
| WB | Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 35-45 kDa (predicted molecular weight: 35 kDa). |
Entrez Gene: 10894 Human
Omim: 605702 Human
SwissProt: Q9Y5Y7 Human
Unigene: 655332 Human
CRSBP1 antibody
extracellular link domain containing 1 antibody
extracellular link domain-containing 1 antibody
Extracellular link domain-containing protein 1 antibody
HAR antibody
Hyaluronic acid receptor antibody
Lymphatic endothelium specific hyaluronan receptor antibody
lymphatic vessel endothelial hyaluronan receptor 1 antibody
Lymphatic vessel endothelial hyaluronic acid receptor 1 antibody
LYVE 1 antibody
LYVE-1 antibody
LYVE1 antibody
LYVE1_HUMAN antibody
XLKD1 antibody
Cell surface retention sequence-binding protein 1 antibody
CRSBP 1 antibody
CRSBP-1 antibody
Immunohistochemistry (Frozen sections) - Anti-LYVE1 antibody (ab10278)
Immunohistochemistry (Frozen sections) analysis of human colon carcinoma tissue sections labelling LYVE1 with ab10278.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody (ab10278)
ab10278 (2µg/ml) staining LYVE1 in human colon using an automated system (DAKO Autostainer Plus). Using this protocol there is lymphatic endothelium staining of lymphatic ducts where blood vessel endothelium and smooth muscle is wholly negative.
Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Western blot - Anti-LYVE1 antibody (ab10278)
All lanes : Anti-LYVE1 antibody (ab10278) at 1 µg/ml
Lane 1 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 35 kDa
Observed band size: 37 kDawhy is the actual band size different from the predicted?
Additional bands at: 22 kDa, 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
LYVE-1 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
Flow Cytometry - Anti-LYVE1 antibody (ab10278)
Flow Cytometry analysis of human dermal microvascular endothelial cells (HDMVEC) labelling LYVE1 with ab10278.
Western blot - Anti-LYVE1 antibody (ab10278)
All lanes : Anti-LYVE1 antibody (ab10278)
Lane 1 : Recombinant Human LYVE1 protein (ab54341)
Lane 2 : Recombinant Mouse LYVE1 protein (ab54342)
Predicted band size: 35 kDa
Immunohistochemistry (Frozen sections) - Anti-LYVE1 antibody (ab10278)This image is courtesy of an Abreview submitted by Dr Vyacheslav Ogay
Rat skin was fixed with paraformaldehyde in 15% saturated picric acid solution for 4hr. Prior to sectioning, the specimen was infiltrated in O.C.T. and frozen in isopentane. The frozen specimen was sectioned these were rinsed in PBS for 15 min to remove O.C.T. and incubated in a 3% sodium deoxycholate solution. The specimens were rinsed twice with distilled water and then with PBS three times. The sections were incubated in 10% normal goat serum for 12 hr at 4°C, then for 12 hr with ab10278. After washing with PBS, the specimens were incubated with Alexa Fluor® 555-conjugated goat anti-rabbit IgG (H+L) (1:500), for 12 hr at 4°C. The cell nuclei were counterstained with YoYo-1. Images were obtained by using confocal microscope.
