Anti-Calnexin抗体
参阅全部 Calnexin 一抗
兔多克隆抗体to Calnexin
Rabbit
Recognizes ER membrane, mitochondria and cis-Golgi
适用于: WBmore details
与反应: Human
Recombinant fragment (His-tag) corresponding to Dog Calnexin aa 1-500. Expressed in E. coli. N-terminal ER luminal domain.
Database link: P24643
WB: Human CEM T-lymphoblastoid leukemia cell lines. MCF cells. HeLa total cell lysate. HeLa membrane fraction. MDA-MB231 total cell lysate. MDA-MB231 membrane fraction.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituent: 99% Rabbit Serum
Whole antiserum
多克隆
IgG
Abpromise™承诺保证使用ab10286于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (14) | 1/2000 - 1/5000. Predicted molecular weight: 67 kDa. 1/2000 - 1/5000. Predicted molecular weight: 67 kDa; however it does run on SDS-PAGE as an ~90kDa protein, this is mainly due to a number of negative charges at the C-terminus of the protein which effects the SDS binding to the molecules and consequently its SDS-PAGE mobility. Read More |
Entrez Gene: 821 Human
Omim: 114217 Human
SwissProt: P27824 Human
Unigene: 567968 Human
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CNX antibody
FLJ26570 antibody
Histocompatibility complex class I antigen binding protein p88 antibody
IP90 antibody
Major histocompatibility complex class I antigen-binding protein p88 antibody
p90 antibody
Calnexin antibody
CALX_HUMAN antibody
CANX antibody
Western blot - Anti-Calnexin antibody (ab10286)
Western blot probed with ab10286 at 1/1000.
Lane 1 – calnexin deficient human NKR T-lymphoblastoid leukemia cell lines.
Lane 2 - human CEM T-lymphoblastoid leukemia cell lines.
Western blot probed with ab10286 at 1/1000. Lane 1 – calnexin deficient human NKR T-lymphoblastoid leukemia cell lines. Lane 2 - human CEM T-lymphoblastoid leukemia cell lines.
Western blot - Anti-Calnexin antibody (ab10286)
Anti-Calnexin antibody (ab10286) at 1/1000 dilution + MCF7 cells
Secondary
Goat anti rabbit at 1/10000 dilution
Predicted band size: 67 kDa
Western blot - Anti-Calnexin antibody (ab10286)This image is courtesy of an Abreview submitted by Dr Neil Taylor
All lanes : Anti-Calnexin antibody (ab10286) at 1/4000 dilution
Lane 1 : HeLa (Total cell lysate)
Lane 2 : HeLa (Membrane fraction)
Lane 3 : HeLa (Cytoplasmic fraction)
Lane 4 : MDA-MB231 (Total cell lysate)
Lane 5 : MDA-MB231 (Membrane fraction)
Lane 6 : MDA-MB231 (Cytoplasmic fraction)
Secondary
All lanes : HRP conjugated donkey anti-rabbit
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 67 kDa
Observed band size: 67 kDa
Exposure time: 5 minutes
Western blot - Anti-Calnexin antibody (ab10286)This image is courtesy of an anonymous Abreview.
Anti-Calnexin antibody (ab10286) at 1/2000 dilution + HeLa whole cell lysate
Secondary
Goat anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 67 kDa
Western blot - Anti-Calnexin antibody (ab10286)This image is courtesy of an Abreview submitted by Mr. Yannick Waumans.
All lanes : Anti-Calnexin antibody (ab10286) at 1/4000 dilution
Lane 1 : CHO membrane fraction
Lane 2 : CHO cytosolic fraction
Secondary
All lanes : HRP-conjugated Goat anti-Rabbit Ig at 1/5000 dilution
Predicted band size: 67 kDa
