Anti-ERp57 antibody

• 产品名称

  Anti-ERp57抗体
  参阅全部 ERp57 一抗

• 描述

  兔多克隆抗体to ERp57

• 宿主

  Rabbit

• 经测试应用

  适用于: ICC/IF, WBmore details

• 种属反应性

  与反应: Mouse, Human
  

• 免疫原

  Recombinant full length protein corresponding to Mouse ERp57.
  Database link: P27773

• 阳性对照

• WB: Mouse embryonic cells.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 纯度

  Whole antiserum

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab10287于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 2923 Human

• Entrez Gene: 14827 Mouse

• Omim: 602046 Human

• SwissProt: P30101 Human

• SwissProt: P27773 Mouse

• Unigene: 591095 Human

• Unigene: 263177 Mouse

• 别名

• Disulfide isomerase ER 60 antibody

• Disulfide isomerase ER-60 antibody

• Endoplasmic reticulum resident protein 57 antibody

• Endoplasmic reticulum resident protein 60 antibody

• ER p57 antibody

• ER protein 57 antibody

• ER protein 60 antibody

• ERp 57 antibody

• ERp57 antibody

• ERp60 antibody

• ERp61 antibody

• Glucose Regulated Protein 58 Kd antibody

• GRP 57 antibody

• GRP 58 antibody

• GRP57 antibody

• HsT17083 antibody

• p58 antibody

• PDIA 3 antibody

• PDIA3 antibody

• PDIA3_HUMAN antibody

• Phospholipase C alpha antibody

• PI PLC antibody

• Protein disulfide isomerase A3 antibody

• Protein disulfide isomerase family A member 3 antibody

• Protein disulfide-isomerase A3 antibody

• 58 kDa glucose regulated protein antibody

• 58 kDa glucose-regulated protein antibody

• 58 kDa microsomal protein antibody

• 

  Western blot - Anti-ERp57 antibody (ab10287)

  All lanes : Anti-ERp57 antibody (ab10287) at 1/1000 dilution
  
  Lane 1 : Mouse embryonic cells
  Lane 2 : Calreticulin deficient mouse embryomic cells
  
  Predicted band size: 61 kDa
  Observed band size: 57 kDawhy is the actual band size different from the predicted?
  
  
  
  

  Western blot using ab10287 at 1/1000.

  Lane 1 – Mouse Embryonic Fibroblasts.
  Lane 2 – Calreticulin deficient Mouse Embryonic Fibroblasts.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody (ab10287)

  Double staining of mouse embryonic fibroblasts (top) and calreticulin deficient fibroblasts (bottom) with rabbit-anti-ERp57 antibodies (1:100;  upper and lower left panel) and FITC-ConA (upper and lower right panel).

• 

  Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody (ab10287)This image is courtesy of an anonymous Abreview

  ab10287 at 1/100 staining human HeLa cells by ICC/IF. The cells were premeabilized with digitonin before being blocked and then stained with the antibody for 1 hour. An Alexa-Fluor ® conjugated goat anti rabbit antibody was used as the secondary.

  See Abreview

• 

  Western blot - Anti-ERp57 antibody (ab10287)

  Anti-ERp57 antibody (ab10287) at 1/1000 dilution + Recombinant Human ERp57 protein (ab92937) at 0.01 µg
  
  Secondary
  Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/5000 dilution
  
  Developed using the ECL technique.
  
  Performed under reducing conditions.
  
  Predicted band size: 61 kDa
  
  
  Exposure time: 150 seconds