Anti-Cardiac Troponin I抗体[4C2]
参阅全部 Cardiac Troponin I 一抗
小鼠单克隆抗体[4C2] to Cardiac Troponin I
Mouse
reacts with free cardiac troponin I (cTnI) and cTnI forming complexes with other troponin components (in the presence of 5 mM EDTA). Not affected by heparin, phosphorylation, oxidation and troponin complex formation. Does not cross-react with skeletal muscle troponin I.
适用于: IHC-Pmore details
与反应: Human
Full length native protein (purified) corresponding to Human Cardiac Troponin I.
23-29 aa
Concentration varies from lot to lot and can be provided on request.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS
浓度
200 µg 浓度为 2 mg/ml
Protein A purified
Purity tested by electrophoresis.
单克隆
4C2
Sp2/0
IgG2a
Abpromise™承诺保证使用ab10231于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (1) | Use a concentration of 1 µg/ml |
Entrez Gene: 7137 Human
Omim: 191044 Human
SwissProt: P19429 Human
Unigene: 709179 Human
Cardiomyopathy, familial hypertrophic, 7, included antibody
CMD1FF antibody
CMD2A antibody
CMH7 antibody
cTnI antibody
Familial hypertrophic cardiomyopathy 7 antibody
MGC116817 antibody
RCM1 antibody
Tn1 antibody
Tni antibody
TNN I3 antibody
TNNC 1 antibody
TNNC1 antibody
TNNI3 antibody
TNNI3_HUMAN antibody
Troponin I antibody
Troponin I cardiac antibody
Troponin I cardiac muscle antibody
Troponin I cardiac muscle isoform antibody
Troponin I type 3 cardiac antibody
troponin I, cardiac 3 antibody
TroponinI antibody
Ttroponin I type 3 (cardiac) antibody
cardiac muscle antibody
Cardiac troponin I antibody
cardiomyopathy, dilated 2A (autosomal recessive) antibody
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [4C2] (ab10231)](https://www.abcam.cn/ps/products/10/ab10231/Images/ab10231-17831-anti-cardiac-troponin-i-antibody-4c2-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [4C2] (ab10231)
Ab10231 staining human heart tissue. Staining is localised to cytoplasm.
Left panel: with primary antibody at 1ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
