Human TGF beta 1 ELISA Kit

Human TGF beta 1 ELISA Kit ab100647 is a sandwich ELISA to measure human TGF beta 1 (TGF-β1) in serum, plasma, and cell culture supernatant with a sensitivity of 18 pg/mL.

How the assay works

This assay employs an antibody specific for Human TGF beta 1 coated on a 96-well plate. Standards and samples are pipetted into the wells and TGF beta 1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TGF beta 1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TGFB 1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Assay specificity

Cross-reactivity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested; ANG, CD23, Eotaxin, GCSF, GM-CSF, GRO-α, GRO-β, GRO-γ, I-309, IFN-γ, IL-1α, IL-1β, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p40), IL-12 (p70), IL-15, IL-16, IP-10, MCP-1, MCP-2, MCP-3, MCP-4, MCSF, MIG, MIP-1α, MIP-1β, NAP-2, PDGF, PF-4, PARC, SCF, SDF-1α, TIMP-1, TIMP-2, TNFβ, TGFβ2, TGFβ3, VEGF.

Human TGF beta 1 ELISA Kit ab100647 protocol summary

1. Add samples and standards to wells. Incubate at room temperature
2. Wash each well and add biotinylated antibody. Incubate at room temperature
3. Wash each well and add Streptavidin Solution. Incubate at room temperature
4. Add TMB Solution to each well. Incubate at room temperature
5. Add Stop Solution to each well. Read immediately

How other researchers are using Human TGF beta 1 ELISA Kit ab100647

Human TGF beta 1 ELISA Kit ab100647 has been used to study inflammation in different physio-pathological contexts such as squamous cell carcinoma (1), gastric cancer (2), and osteoarthritis (3).

References:

(1)Yi Huang et al. 2023, PMID: 37872157,
(2)Shuai Lv et al. 2024, PMID: 38490994,
(3)Zhong Wu, et al. 2023, PMID: 37842584

Related and recommended products

Human TGF beta 1 ELISA Kit ab100647 is commonly used to measure IFN gamma as a marker of inflammation.

Other ELISA kits to measure inflammatory markers include:

- Human IL-6 ELISA kit ab178013
- Human IL-10 ELISA Kit (Interleukin-10) ab185986
- Human TNF alpha ELISA kit ab181421